Immunohistochemical analysis on MMP-2 and MMP-9 for wound age determination
Immunohistochemical analysis on MMP-2 and MMP-9 for wound age determination
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DOI：
10.1007/s00414-015-1167-5
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发表时间：
2015-09-01
影响因子：
2.1
通讯作者：
Kondo, Toshikazu
中科院分区：
文献类型：
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作者：
Ishida, Yuko;Kuninaka, Yumi;Kondo, Toshikazu
我们采用免疫组化和形态计量学相结合的方法检测了55例不同年龄的人皮肤创面中基质金属蛋白酶-2 （MMP-2）和MMP-9的表达：I组，0-3 d (n = 16)；II， 4-7天（n = 11）；III， 9-14天（n = 16）；IV期17 ~ 21 d （n = 12）。MMP-2免疫阳性反应在所有人体皮肤标本中观察到，包括未损伤皮肤作为对照。MMP-2（+）巨噬细胞数量随伤口年龄的增加而显著增加。与MMP-2相比，在未损伤和创伤小于1天的标本中未检测到MMP-9（+）信号。然而，MMP-9（+）巨噬细胞的数量在II组和III组大量出现。形态学上，在9 ~ 12天龄的所有创面样本中，MMP-2（+）细胞数量均大于20个。相反，大多数剩余样品的阳性细胞数< 20。然而，只有一个样本（一个7天的伤口）显示21个阳性细胞。因此，从法医安全的实际适用性来看，bbb20的MMP-2（+）巨噬细胞表明伤口年龄为7-12天。另外，在12个9-12天的创面标本中，有10个标本显示> 25的MMP-2（+）细胞数，提示> 25的MMP-2（+）细胞数为9-12天的创面年龄。相反，所有3-14 d的创面样本中，除了只有一个样本的MMP-9（+）细胞数量为> 30，表明MMP-9（+）细胞数量为> 30，表明创面年龄为3-14 d。总的来说，与MMP-9相比，MMP-2似乎是更明显的标志物。
We performed immunohistochemical study combined with morphometrical analyses in order to examine the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9 using 55 human skin wounds of different ages: group I, 0-3 days (n = 16); II, 4-7 days (n = 11); III, 9-14 days (n = 16); and IV, 17-21 days (n = 12). Immunopositive reactions for MMP-2 were observed in all human skin specimens including uninjured skin as control. The number of MMP-2(+) macrophages was significantly increased in accordance with wound ages. In contrast to MMP-2, no MMP-9(+) signals were detected in uninjured and wound specimens aged less than 1 day. However, the number of MMP-9(+) macrophages profoundly appeared in groups II and III. Morphometrically, in all of wound samples aged 9-12 days, MMP-2(+) cell number was more than 20. On the contrary, most of the remaining samples had < 20 positive cells. However, only one sample (a 7-day-old wound) showed 21 positive cells. Thus, with regard to practical applicability with forensic safety, MMP-2(+) macrophages of > 20 would indicate a wound age of 7-12 days. Additionally, 10 out of 12 wound specimens aged 9-12 days showed the MMP-2(+) cell number of > 25, implying that MMP-2(+) cell number of > 25 would indicate the wound age of 9-12 days. On the contrary, all wound samples aged 3-14 days except for only one sample had MMP-9(+) cell number of > 30, indicating that MMP-9(+) cell number of > 30 would indicate the wound age of 3-14 days. Collectively, MMP-2 seemed to be more distinct marker, compared with MMP-9.