Mutations in the Escherichia coli surE gene increase isoaspartyl accumulation in a strain lacking the pcm repair methyltransferase but suppress stress-survival phenotypes.
Mutations in the Escherichia coli surE gene increase isoaspartyl accumulation in a strain lacking the pcm repair methyltransferase but suppress stress-survival phenotypes.
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大肠杆菌 surE 基因突变会增加缺乏 pcm 修复甲基转移酶的菌株中异天冬氨酰的积累，但会抑制应激生存表型。
DOI：
10.1111/j.1574-6968.1998.tb13202.x
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发表时间：
1998
影响因子：
2.1
通讯作者：
Clarke,S
中科院分区：
文献类型：
--
作者：
Visick,JE;Ichikawa,JK;Clarke,S
大肠杆菌surE基因与pcm共转录，编码β-异戊酰蛋白修复甲基转移酶，并且在真细菌和大肠杆菌中高度保守;然而，尚未确定该基因的生化功能。apcm surE双突变体在稳定期的异戊酰积累量明显高于单突变体，表明这两个基因可能代表了两条平行的途径，通过这两条途径，E.大肠杆菌多糖对蛋白质损伤作出反应。无效突变insurE还抑制了之前在apcm突变株中观察到的应激生存缺陷，为两种基因产物之间的相互作用提供了进一步的证据。
TheEscherichia coli surEgene is co-transcribed withpcm, encoding thel-isoaspartyl protein repair methyltransferase, and is highly conserved among both the Eubacteria and the Archaea; however, no biochemical function has yet been identified for this gene. Isoaspartyl accumulation during stationary phase was much higher in apcm surEdouble mutant than in either single mutant, suggesting that the two genes may represent two parallel pathways by whichE. colican respond to protein damage. A null mutation insurEalso suppressed stress-survival defects previously observed in apcmmutant strain, providing further evidence for an interaction between the two gene products.