Both GA2, GM2, and GD2 synthases and GM1b, GD1a, and GT1b synthases are single enzymes in Golgi vesicles from rat liver.
Both GA2, GM2, and GD2 synthases and GM1b, GD1a, and GT1b synthases are single enzymes in Golgi vesicles from rat liver.
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GA2、GM2 和 GD2 合酶以及 GM1b、GD1a 和 GT1b 合酶都是大鼠肝脏高尔基体囊泡中的单酶。
DOI：
10.1073/pnas.85.19.7044
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发表时间：
1988
影响因子：
11.1
通讯作者：
K. Sandhoff
中科院分区：
文献类型：
--
作者：
Gottfried Pohlentz;D. Klein;Gunter Schwarzmann;Doris Schmitz;K. Sandhoff
竞争实验使用乳糖神经酰胺，神经节苷脂GM 3和神经节苷脂GD 3作为底物，以及相互抑制神经节苷脂N-乙酰半乳糖胺转移酶，在高尔基体囊泡来自大鼠肝脏表明，N-乙酰半乳糖胺转移到这三个各自的化合物，导致神经节苷脂GA 2，GM 2，和GD 2，分别是由一种酶催化。在唾液酸转移酶测定中使用神经节苷脂GA 1、GM 1和GD 1b作为糖脂受体的类似研究表明GM 1b、GD 1a和GT 1b合酶是相同的。这些结果被纳入神经节苷脂的生物合成及其调控的模型。
Competition experiments using lactosylceramide, ganglioside GM3 and ganglioside GD3 as substrates, as well as mutual inhibitors for ganglioside N-acetylgalactosaminyltransferase, in Golgi vesicles derived from rat liver suggested that N-acetylgalactosamine transfer to these three respective compounds, leading to gangliosides GA2, GM2, and GD2, respectively, is catalyzed by one enzyme. Analogous studies with gangliosides GA1, GM1, and GD1b as glycolipid acceptors in sialyltransferase assays indicated GM1b, GD1a, and GT1b synthases to be identical. These results are incorporated into a model for ganglioside biosynthesis and its regulation.