Nitrogen-doped carbon quantum dots as a fluorescent probe to detect copper ions, glutathione, and intracellular pH
Nitrogen-doped carbon quantum dots as a fluorescent probe to detect copper ions, glutathione, and intracellular pH
复制标题
氮掺杂碳量子点作为荧光探针检测铜离子、谷胱甘肽和细胞内 pH
DOI：
10.1007/s00216-018-1387-x
复制
发表时间：
2018-11-01
影响因子：
4.3
通讯作者：
Chen, Xiaoqing
中科院分区：
文献类型：
--
作者：
Liao, Sen;Huang, Xueqian;Chen, Xiaoqing
开发了一种简便的一步水热法，利用六亚甲基四胺作为碳源和氮源合成氮掺杂碳量子点（N-CQD）。激发波长为 420 nm 时，量子产率 (QY) 为 21.7%，最大发射波长为 508 nm。这种 N-CQD 荧光探针已成功应用于选择性测定铜离子 (Cu2+) 的浓度，线性范围为 0.1–40 μM，检测限为 0.09 μM。此外，N-CQDs的荧光可以被Cu2+有效猝灭，并被谷胱甘肽（GSH）特异性恢复，这使得N-CQDs成为检测GSH的优质荧光探针。该荧光“开启”方案用于测定 GSH，线性范围为 0.1–30 μM，检测限为 0.05 μM。对于pH检测，在2.87-7.24的pH范围内具有良好的线性。此外，N-CQD 是一种有前途且方便的荧光 pH、Cu2+ 和谷胱甘肽传感器，在环境监测和生物成像应用中具有出色的生物相容性和低细胞毒性。
A facile one-step hydrothermal method was developed to synthesize nitrogen-doped carbon quantum dots (N-CQDs) by utilizing hexamethylenetetramine as the carbon and nitrogen source. The quantum yield (QY) of 21.7% was under the excitation wavelength of 420 nm with maximum emission at 508 nm. This N-CQD fluorescent probe has been successfully applied to selectively determine the concentration of copper ion (Cu2+) with a linear range of 0.1–40 μM and a limit of detection of 0.09 μM. In addition, the fluorescence of N-CQDs could be effectively quenched by Cu2+and specifically recovered by glutathione (GSH), which render the N-CQDs as a premium fluorescent probe for GSH detection. This fluorescence “turn-on” protocol was applied to determine GSH with a linear range of 0.1–30 μM as well as a detection limit of 0.05 μM. For pH detection, there is good linearity in the pH range of 2.87–7.24. Furthermore, N-CQD is a promising and convenient fluorescent pH, Cu2+, and glutathione sensor with brilliant biocompatibility and low cytotoxicity in environmental monitoring and bioimaging applications.