QTL Mapping of Kernel Number-Related Traits and Validation of One Major QTL for Ear Length in Maize.
QTL Mapping of Kernel Number-Related Traits and Validation of One Major QTL for Ear Length in Maize.
复制标题
玉米籽粒数相关性状的 QTL 定位及穗长的一个主要 QTL 的验证
DOI：
10.1371/journal.pone.0155506
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Huo D;Ning Q;Shen X;Liu L;Zhang Z
出粒数是粮食产量的组成部分，也是玉米育种的重要目标。穗长、每行粒数、穗行数与单穗粒数高度相关，最终决定穗重和籽粒产量。在本研究中，利用共享一个自交系的两个双亲杂交产生的两组 F2:3 家族来鉴定四个与粒数相关的性状的数量性状位点 (QTL)：穗长、每行粒数、穗行数和穗重。在两个群体中共鉴定出这四个性状的 39 个 QTL。由单个QTL解释的表型变异范围为0.4%至29.5%。此外，14 个重叠的 QTL 在 1、4、5、7 和 10 号染色体上形成 5 个 QTL 簇。有趣的是，6 个与穗长和每行籽粒数相关的 QTL 在 1 号染色体的一个区域中重叠。该区域被指定为 qEL1.10，并被验证为同时负责近等基因系衍生群体中的穗长、每行籽粒数和穗重，这表明 表明qEL1.10是一个效应较大的多效性QTL。此外，6个优良自交系与qEL1.10的两个近等基因系杂交产生的杂交体的性能显示了qEL1.10对于提高玉米杂交种的出粒数和籽粒产量的育种价值。该研究为玉米籽粒数相关性状的进一步精细定位、分子标记辅助育种和功能研究提供了基础。
The kernel number is a grain yield component and an important maize breeding goal. Ear length, kernel number per row and ear row number are highly correlated with the kernel number per ear, which eventually determines the ear weight and grain yield. In this study, two sets of F2:3 families developed from two bi-parental crosses sharing one inbred line were used to identify quantitative trait loci (QTL) for four kernel number-related traits: ear length, kernel number per row, ear row number and ear weight. A total of 39 QTLs for the four traits were identified in the two populations. The phenotypic variance explained by a single QTL ranged from 0.4% to 29.5%. Additionally, 14 overlapping QTLs formed 5 QTL clusters on chromosomes 1, 4, 5, 7, and 10. Intriguingly, six QTLs for ear length and kernel number per row overlapped in a region on chromosome 1. This region was designated qEL1.10 and was validated as being simultaneously responsible for ear length, kernel number per row and ear weight in a near isogenic line-derived population, suggesting that qEL1.10 was a pleiotropic QTL with large effects. Furthermore, the performance of hybrids generated by crossing 6 elite inbred lines with two near isogenic lines at qEL1.10 showed the breeding value of qEL1.10 for the improvement of the kernel number and grain yield of maize hybrids. This study provides a basis for further fine mapping, molecular marker-aided breeding and functional studies of kernel number-related traits in maize.