Effects of nanomolar copper on water plants--comparison of biochemical and biophysical mechanisms of deficiency and sublethal toxicity under environmentally relevant conditions.
Effects of nanomolar copper on water plants--comparison of biochemical and biophysical mechanisms of deficiency and sublethal toxicity under environmentally relevant conditions.
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纳摩尔铜对水生植物的影响--环境相关条件下缺乏和亚致死毒性的生化和生物物理机制比较
DOI：
10.1016/j.aquatox.2013.05.008
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发表时间：
2013
影响因子：
4.5
通讯作者：
Küpper H
中科院分区：
文献类型：
--
作者：
Thomas G;Stärk H-J;Wellenreuther G;Dickinson BC;Küpper H
铜等人体必需微量元素的毒性和缺乏是全球性的重大问题。在这里，环境相关的亚微摩尔浓度的铜（提供硫酸铜）和模拟自然光和温度循环应用于水生植物金鱼藻。生长在10 nM Cu时最佳，而PSII活性（Fv/Fm）在2 nM Cu左右最大。对PSII反应中心的损害是铜毒性的第一目标，其次是热耗散（NPQ）的干扰调节。只有在此之后，通过PSII的电子传递（ΦPSII）受到抑制，最终叶绿素下降。铜在植物中的积累是稳定的，直到10纳米铜溶液中，但强烈增加，在更高的浓度。静脉是铜的主要存储站点高达生理浓度（10 nM）。在毒性水平，它也被隔离到表皮和叶肉，直到出口从静脉成为抑制，伴随着抑制锌的吸收。6周后，铜缺乏导致生长在“0”nM Cu时完全停止。这是伴随着高淀粉积累，虽然电子流通过PSII（ΦPSII）从2周开始下降，其次是减少色素和增加非光化学猝灭（NPQ）。从植物释放铜低于10纳米铜供应的营养液中表示缺乏高亲和力的铜转运蛋白，并在组织水平上铜缺乏导致锌的重新分配。
Toxicity and deficiency of essential trace elements like Cu are major global problems. Here, environmentally relevant sub-micromolar concentrations of Cu (supplied as CuSO4) and simulations of natural light- and temperature cycles were applied to the aquatic macrophyteCeratophyllum demersum. Growth was optimal at 10 nM Cu, while PSII activity (Fv/Fm) was maximal around 2 nM Cu. Damage to the PSII reaction centre was the first target of Cu toxicity, followed by disturbed regulation of heat dissipation (NPQ). Only after that, electron transport through PSII (ΦPSII) was inhibited, and finally chlorophylls decreased. Copper accumulation in the plants was stable until 10 nM Cu in solution, but strongly increased at higher concentrations. The vein was the main storage site for Cu up to physiological concentrations (10 nM). At toxic levels it was also sequestered to the epidermis and mesophyll until export from the vein became inhibited, accompanied by inhibition of Zn uptake. Copper deficiency led to a complete stop of growth at “0” nM Cu after 6 weeks. This was accompanied by high starch accumulation although electron flow through PSII (ΦPSII) decreased from 2 weeks, followed by decrease in pigments and increase of non photochemical quenching (NPQ). Release of Cu from the plants below 10 nM Cu supply in the nutrient solution indicated lack of high-affinity Cu transporters, and on the tissue level copper deficiency led to a re-distribution of zinc.
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影响因子：
15
作者：
Dickinson BC;Huynh C;Chang CJ
通讯作者：
Chang CJ
影响因子：
10.1
作者：
A. McIntosh
通讯作者：
A. McIntosh
影响因子：
1.7
作者：
De Nolf, Wout;Janssens, Koen
通讯作者：
Janssens, Koen
C.E.B.A.S.-AQUARACK 项目：迷你模块作为人工生态系统研究的工具。
影响因子：
3.5
作者：
V. Blum;E. Stretzke;K. Kreuzberg
通讯作者：
K. Kreuzberg
影响因子：
4.3
作者：
LIDON, FC;HENRIQUES, FS
通讯作者：
HENRIQUES, FS