High-efficiency Generation of Multiple Short Noncoding RNA in B-cells and B-cell-derived Extracellular Vesicles.
High-efficiency Generation of Multiple Short Noncoding RNA in B-cells and B-cell-derived Extracellular Vesicles.
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DOI：
10.1038/mtna.2015.44
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发表时间：
2015-12-15
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影响因子：
--
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短非编码（snc）RNA是具有治疗潜力的生物制剂领域的重要新成员。最近，我们报道了一种新的方法，用于在B细胞转染质粒DNA的snc RNA的合成和交付。在这里，使用相同的方法，我们证明了B细胞可以被编程为强制生物合成和同步释放多个sncRNA。我们的数据表明，这一目标是可行的，并且多个sncRNA在细胞外区室中释放的量与编程为仅表达和分泌一种scnRNA的B细胞的量相当。此外，我们发现从程序化B细胞分离的细胞外囊泡（EV）的货物显著富集多个sncRNA。平均而言，我们发现EV中多个sncRNA的含量为3.6拷贝数/EV。总的来说，我们证明了B细胞可以很容易地被编程为有效和特异地合成和释放多种sncRNA，包括装载sncRNA的EV。
Short noncoding (snc)RNAs are important new players in the landscape of biologics with therapeutic potential. Recently, we reported on a new method for the synthesis and delivery of snc RNA in B-cells transfected with plasmid DNA. Here using the same approach, we demonstrate that B-cells can be programmed for the enforced biogenesis and synchronous release of multiple sncRNAs. Our data show that this goal is feasible and that multiple sncRNA are released in the extracellular compartment in amounts comparable to those from B-cells programmed to express and secrete one scnRNA only. Furthermore, we found that the cargo of extracellular vescicles (EVs) isolated from programmed B-cells is remarkably enriched for multiple sncRNA. On average, we found that the content of multiple sncRNAs in EVs is 3.6 copynumber/EV. Collectively, we demonstrate that B-cells can be easily programmed toward the synthesis and release of multiple sncRNAs, including sncRNA-laden EVs, efficiently and specifically.
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影响因子：
46.9
作者：
Alvarez-Erviti, Lydia;Seow, Yiqi;Wood, Matthew J. A.
通讯作者：
Wood, Matthew J. A.
影响因子：
64.5
作者：
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影响因子：
3.7
作者：
Chen WX;Liu XM;Lv MM;Chen L;Zhao JH;Zhong SL;Ji MH;Hu Q;Luo Z;Wu JZ;Tang JH
通讯作者：
Tang JH
影响因子：
16
作者：
Bogerd HP;Karnowski HW;Cai X;Shin J;Pohlers M;Cullen BR
通讯作者：
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DOI：
10.1073/pnas.1311145110
发表时间：
2013-12-10
影响因子：
11.1
作者：
Almanza, Gonzalo;Anufreichik, Veronika;Zanetti, Maurizio
通讯作者：
Zanetti, Maurizio