Monitoring of metabolic responses of intact Haliotis (abalones) under salinity stress by 31P surface probe localized NMR.
Monitoring of metabolic responses of intact Haliotis (abalones) under salinity stress by 31P surface probe localized NMR.
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通过 31P 表面探针局部 NMR 监测盐度胁迫下完整鲍鱼的代谢反应。
DOI：
10.1002/jez.1402490317
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Macdonald,JM
中科院分区：
文献类型：
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作者：
Higashi,RM;Fan,TW;Macdonald,JM
采用表面探针定位的31p核磁共振波谱法，记录了完整爪爪和爪爪的代谢反应。高压和低渗胁迫下的黑鲍鱼和红鲍鱼。使用表面探针可以对完整鲍鱼的足部进行光谱定位，方便了对不同大小动物的监测，并最大限度地减少了均匀场探针通常对水生室设计的限制。一般来说，高渗应激（51‰）比低渗应激（17‰）引起的磷酸盐代谢物变化更快。与在完整哺乳动物和切除的软体动物组织中进行的充分研究的缺氧应激一样，两种盐度处理都导致了磷酸盐的下降和无机磷酸盐水平的增加。然而，渗透胁迫不同于缺氧胁迫，细胞内pH值没有变化，三磷酸核苷酸（NTP）浓度立即下降。虽然这些发现是初步的，但它们证明了表面探针方法在完整海洋无脊椎动物环境应力研究中的实用性。
Surface probe localized31P NMR spectroscopy was employed to record the metabolic responses of the foot of intactHaliotis cracherodiiandH. rufescens(black and red abalones) under hyper‐ and hypoosmotic stresses. Use of the surface probe allowed spectral localization on the foot of intact abalones, facilitated monitoring of different sizes of animals, and minimized constraints on aquatic chamber design normally imposed by homogeneous‐field probes. Generally, hyperosmotic stress (51‰) elicited more rapid changes of phosphate metabolites than hypoosmotic stress (17‰). As with the well‐studied hypoxic stress in intact mammalian and excised molluscan tissue, both salinity treatments caused drops in the phosphagen and increases in inorganic phosphate levels. However, osmotic stress was distinct from hypoxic stress inthat intracellular pH did not change and nucleotide triphosphate (NTP) concentrations dropped immediately. Although these findings are preliminary, they demonstrate the utility of the surface probe approach for studies of environmental stress in intact marine invertebrates.