Short-RNA selective binding of oligonucleotides modified using adenosine and guanosine derivatives that possess cyclohexyl phosphates as substituents.
Short-RNA selective binding of oligonucleotides modified using adenosine and guanosine derivatives that possess cyclohexyl phosphates as substituents.
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使用具有环己基磷酸酯作为取代基的腺苷和鸟苷衍生物修饰的寡核苷酸的短RNA选择性结合。
DOI：
10.1039/c1ob06580g
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发表时间：
2012
影响因子：
3.2
通讯作者：
M. Sekine
中科院分区：
文献类型：
--
作者：
K. Seio;Sayako Kurohagi;Erika Kodama;Yoshiaki Masaki;Hirosuke Tsunoda;A. Ohkubo;M. Sekine
我们开发了新的人工寡核苷酸，可以区分短RNA靶标和长RNA靶标。通过使用腺苷衍生物修饰寡核苷酸的5‘端，这些衍生物在其碱基部分具有较大的环己基磷酸基团，在其5’-羟基位置具有磷酸基团，从而最大化了它们的短RNA选择性。2'- o -甲基RNA (5'-XC(m)A(m)A(m)C(m)C(m)U(m)A(m)C(m)U(m) A(m)C（m)U(m)）与互补短RNA （3'- guugauga -5‘）的双双体相比，具有这些修饰的长RNA （3’- auuauaugugaugaugua -5'）的T(m)高约10°C。具有相同修饰的寡脱氧核苷酸表现出相似的选择性。这种末端修饰寡核苷酸的短rna选择性结合可以用来区分成熟的microrna和pre- microrna。
We have developed new artificial oligonucleotides which distinguish short RNA targets from long ones. The modification of the 5' termini of oligonucleotides by using adenosine derivatives that possess a bulky cyclohexyl phosphate moiety at their base moiety and a phosphate group at the position of their 5'-hydroxyl group maximized their short RNA selectivity. The 2'-O-methyl-RNA (5'-XC(m)A(m)A(m)C(m)C(m)U(m)A(m)C(m)U(m)) having these modifications exhibits ca. 10 °C higher T(m) in the duplexes with the complementary short RNA (3'-GUUGGAUGA-5') than with the long RNA (3'-AUUAUAUGUUGGAUGAUGGUUA-5'). The oligodeoxynucleotides having the same modification exhibited similar selectivity. Such short-RNA selective binding of terminally modified oligonucleotides can be employed to distinguish between mature microRNAs and pre-microRNAs.
影响因子：
3.4
作者：
Qing Zhang;T. Schlick
通讯作者：
Qing Zhang;T. Schlick
核寡核苷酸末端错配能量学的序列依赖性。
影响因子：
2.9
作者：
Sugimoto,N;Kierzek,R;Turner,DH
通讯作者：
Turner,DH
影响因子：
2.9
作者：
FREIER, SM;KIERZEK, R;TURNER, DH
通讯作者：
TURNER, DH