Cooperative Hybridization of Oligonucleotides
Cooperative Hybridization of Oligonucleotides
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DOI：
10.1021/ja109089q
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发表时间：
2011-02-02
影响因子：
15
通讯作者：
Zhang, David Yu
中科院分区：
文献类型：
--
作者：
Zhang, David Yu
核酸已被证明是多功能的纳米级工程材料，具有构建动态DNA结构、马达和电路的功能。这些构造通常依赖于相对较少的反应机制和设计基元的巧妙使用和集成。在此，引入协同杂交作为一种机制，其中两个独立序列的寡核苷酸可以化学计量地、同时地和协同地与DNA复合物杂交。合作杂交严格的特点和建模，并实现数字浓度比较放大，以及数字布尔逻辑。这些设计基于协同杂交，擅长于对杂质的稳健性，并且不需要寡核苷酸纯化。
Nucleic acids have been demonstrated to be versatile nanoscale engineering materials with the construction of dynamic DNA structures, motors, and circuits. These constructions generally rely on the clever use and integration of relatively few reaction mechanisms and design primitives. Here, cooperative hybridization is introduced as a mechanism in which two oligonucleotides of independent sequence can stoichiometrically, simultaneously, and cooperatively hybridize to a DNA complex. Cooperative hybridization is rigorously characterized and modeled and is shown to implement digital concentration comparison with amplification, as well as digital Boolean logic. These designs, based on cooperative hybridization, excel in being robust to impurities and not requiring oligonucleotide purification.