High-Fidelity Modelling Methodology of Light-Limited Photosynthetic Production in Microalgae.
High-Fidelity Modelling Methodology of Light-Limited Photosynthetic Production in Microalgae.
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DOI：
10.1371/journal.pone.0152387
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Bezzo F
中科院分区：
文献类型：
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作者：
Bernardi A;Nikolaou A;Meneghesso A;Morosinotto T;Chachuat B;Bezzo F
对微藻光限制生长的可靠定量描述是改进工业生产系统的设计和操作的关键。本文展示了如何利用系统化的实验技术设计，利用数学建模和实验室规模实验之间的协同作用来预测光合作用过程。作者开发的叶绿素荧光模型 [Nikolaou et al., J Biotechnol 194:91–99, 2015] 被用作起点，从而对非光化学猝灭 (NPQ) 过程的表示进行了改进，以实现生物一致性。该模型跨越从毫秒到小时的多个时间尺度，因此需要结合各种实验技术，以获得足够丰富的数据集并确定模型参数的统计上有意义的估计。该方法通过结合脉冲幅度调制 (PAM) 荧光、光合作用速率和天线尺寸测量，针对微藻微绿球藻进行了验证。结果表明，校准模型能够在各种瞬态光条件下进行准确的定量预测。此外，这项工作还为理论化的荧光和光合作用-辐照度（PI）曲线之间的联系提供了实验验证。
Reliable quantitative description of light-limited growth in microalgae is key to improving the design and operation of industrial production systems. This article shows how the capability to predict photosynthetic processes can benefit from a synergy between mathematical modelling and lab-scale experiments using systematic design of experiment techniques. A model of chlorophyll fluorescence developed by the authors [Nikolaou et al., J Biotechnol 194:91–99, 2015] is used as starting point, whereby the representation of non-photochemical-quenching (NPQ) process is refined for biological consistency. This model spans multiple time scales ranging from milliseconds to hours, thus calling for a combination of various experimental techniques in order to arrive at a sufficiently rich data set and determine statistically meaningful estimates for the model parameters. The methodology is demonstrated for the microalga Nannochloropsis gaditana by combining pulse amplitude modulation (PAM) fluorescence, photosynthesis rate and antenna size measurements. The results show that the calibrated model is capable of accurate quantitative predictions under a wide range of transient light conditions. Moreover, this work provides an experimental validation of the link between fluorescence and photosynthesis-irradiance (PI) curves which had been theoricized.