Heparin and rosuvastatin calcium-loaded poly-(L-lactide-co-caprolactone) nanofiber-covered stent-grafts for aneurysm treatment
Heparin and rosuvastatin calcium-loaded poly-(L-lactide-co-caprolactone) nanofiber-covered stent-grafts for aneurysm treatment
复制标题
用于动脉瘤治疗的肝素和瑞舒伐他汀载钙聚（L-丙交酯-己内酯）纳米纤维覆盖覆膜支架
DOI：
10.1039/c7nj01214d
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发表时间：
2017-09-07
影响因子：
3.3
通讯作者：
Mo, Xiumei
中科院分区：
文献类型：
--
作者：
Feng, Wenhao;Liu, Peixi;Mo, Xiumei
血栓形成引起的呼吸困难是血管内支架植入的最大障碍之一。支架管腔的快速内皮化是预防血栓形成的有希望的方法。通过同轴静电纺丝制备了一种覆盖有肝素和瑞舒伐他汀载钙P（LLA-CL）纳米纤维的覆膜支架，作为一种新型的覆膜支架。用扫描电镜和透射电镜分别观察了核壳结构纳米纤维的形貌和内部结构。研究了该材料的力学性能、水接触角、瑞舒伐他汀钙的释放特性、细胞粘附和增殖以及体外抗凝性能。结果表明，肝素和瑞舒伐他汀钙成功地包裹在纳米纤维基质中，并且依靠P（LLA-CL）同轴纳米纤维的优异机械性能，它不会随着覆膜支架的扩张而破裂。核-壳纳米纤维具有均匀光滑的形貌。负载在P（LLA-CL）同轴纳米纤维内的瑞舒伐他汀钙显示出持续释放曲线。由于瑞舒伐他汀钙的存在，HUVECs可以在这些电纺纳米纤维上生长增殖良好，表明这种材料具有良好的细胞相容性。抗凝血实验表明，该材料具有显著的抗凝血能力。将该覆膜支架植入家兔模型中，以测试支架植入后是否可以闭塞动脉瘤。这项工作提供了一个有前途的方法来制造支架移植物动脉瘤治疗。
Restenosis caused by thrombopoiesis is one of the biggest hindrances to endovascular stent-grafts. Rapid endothelialization of the lumen of the stent is a promising approach to prevent thrombosis. A stent-graft covered with heparin and rosuvastatin calcium-loaded P(LLA-CL) nanofibers via coaxial electrospinning has been fabricated as a novel type of stent-graft. The morphology and inner-structure of the core-shell nanofibers were respectively observed by scanning electron microscopy and transmission electron microscopy. The mechanical properties, water contact angle, release profile of rosuvastatin calcium, cell adhesion and proliferation, and anticoagulation properties in vitro were investigated. The results showed that heparin and rosuvastatin calcium were successfully encapsulated in the nanofibrous matrix, and it would not rupture with the expansion of the stent-graft, relying on the excellent mechanical properties of the P(LLA-CL) coaxial nanofibers. The core-shell nanofibers exhibited a uniform and smooth morphology. The rosuvastatin calcium loaded within the P(LLA-CL) coaxial nanofibers showed a sustained release profile. Because of the existence of rosuvastatin calcium, HUVECs can grow and proliferate well on these electrospun nanofibers, indicating that this material has good cytocompatibility. Furthermore, the anticoagulation experiment shows that this material has a remarkable anticoagulation ability. This stent-graft will be implanted in a rabbit model to test whether aneurysms can be obliterated after stenting. This work provides a promising approach to fabricate stent-grafts for aneurysm treatment.