Effects of feeding states on lipid radioactivity in liver, muscle and adipose tissue after injection of labelled glucose in the rat.
Effects of feeding states on lipid radioactivity in liver, muscle and adipose tissue after injection of labelled glucose in the rat.
复制标题
喂养状态对大鼠注射标记葡萄糖后肝脏、肌肉和脂肪组织脂质放射性的影响。
DOI：
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发表时间：
1970
期刊：
影响因子：
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通讯作者：
Z. Somlo
中科院分区：
文献类型：
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作者：
P. Björntorp;M. Krotkiewski;B. Larsson;Z. Somlo
将0.5 g葡萄糖/kg b.w.和（U-14C）葡萄糖静脉注射给自由喂养、禁食18小时或禁食48小时的大鼠。60分钟后血糖降至注射前水平。血液中的丙酮酸-乳酸和少量甘油-甘油中发现放射性代谢物的再循环。此时处死大鼠，用氯仿：肌肉、肝脏和不同脂肪组织区域匀浆的甲醇提取物测量放射性。此外，还测量了脂肪酸的放射性。脂肪酸在不同区域的放射性被认为是由于脂肪酸的局部合成，因为在分析时没有发现再循环。肝脏和脂肪组织脂肪酸合成在饲料大鼠中最为明显。自由饲养大鼠脂肪组织的脂肪酸合成活性高于肝脏，而饲养大鼠没有。甘油-甘油在肌肉中的放射性相当高。在脂肪组织中，在不同饲养状态下，它比脂肪酸放射性更稳定。通过添加测量的放射性代谢物，脂肪组织中的葡萄糖摄取大致估计为总葡萄糖摄取的百分之几。
0.5 g of glucose/kg b.w. and (U-14C) glucose was injected i.v. to ad libitum fed, 18 hrs fasting or 48 hrs fasting-48 hrs refed rats. After 60 min blood glucose had decreased to preinjection values. Recirculation of radioactive metabolites was found in blood in pyruvate-lactate and to a small extent in glyceride-glycerol. Rats were sacrificed at this time and radioactivity measured in chloroform: methanol extracts of homogenates from muscle, liver and different adipose tissue regions. Furthermore, radioactivity was measured in fatty acids. Fatty acid radioactivity in different regions was considered to be due to the local synthesis of fatty acids, because no recirculation was discovered at the time of analysis. Liver and adipose tissue fatty acid synthesis was most pronounced in the refed rats. Adipose tissue showed higher fatty acid synthesizing activity than the liver in ad libitum fed, but not in refed rats. Glyceride-glycerol radioactivity was rather high in muscle. In adipose tissue it was more constant in different feeding states than fatty acid radioactivity was. By additions of measured radioactive metabolites glucose uptake in adipose tissue was roughly estimated to a few per cent of total glucose uptake.