Genetic Diversity of Transcriptional Activator-Like Effector Genes in Chinese Isolates of Xanthomonas oryzae pv. oryzicola
Genetic Diversity of Transcriptional Activator-Like Effector Genes in Chinese Isolates of Xanthomonas oryzae pv. oryzicola
复制标题
米黄单胞菌中国分离株转录激活因子样效应基因的遗传多样性。
DOI：
10.1094/phyto-08-13-0232-r
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发表时间：
2014-07-01
期刊：
影响因子：
3.2
通讯作者：
Chen, Gong-You
中科院分区：
文献类型：
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作者：
Ji, Zhi-Yuan;Zakria, Muhammad;Chen, Gong-You
水稻白稻瘟病菌引起细菌性条斑病（BLS），这是亚洲国家水稻的一种毁灭性病害。X.耳霉致病变种稻瘟病菌利用转录激活子样效应子（TALEs）的全部功能来操纵宿主的抗性或易感性;因此，TALEs可以决定BLS的结果。本研究对65 X.耳霉致病变种wyzico/一种源自中国南方九个省的菌株。用BamHI酶切65个菌株的基因组DNA，并与avrXa 3的内部片段杂交，avrXa 3是来源于相关病原体X.耳霉致病变种稻瘟病（otyzae），其引起细菌性叶枯病（BLB）。Southern印迹分析表明所述菌株含有可变数目（9至22）的avrXa 3-杂交片段（例如，推定的TALL基因）。根据杂交带的数目和大小，菌株可分为14种基因型（命名为1 - 14），其中基因型3和10分别占总数的29.23%和24.64%。在14种基因型中有12种存在高分子量BamHI片段（HMWB;约6.0kb），并且HMWB的序列分析揭示了存在C末端截短的tale、与IS 1403相关的插入元件、编码磷酸甘油酸变位酶和核酸内切酶V的基因。oryzaepv.稻瘟菌属菌株的PCR扩增。X.毒力耳霉致病变种在23个含有不同BLB抗性基因的水稻品种上对稻瘟病菌进行了鉴定。副艇长oryzaepv.稻瘟病菌可分为14个致病型（Ⅰ ~ Ⅹ Ⅳ），菌株的分组与基因型分析所确定的类型基本一致。一般来说，含有较高数目的推定tale基因的菌株比含有较少tale基因的菌株对水稻更具毒性。结果还表明，供试水稻品系与X. oryzaepv.稻生菌属菌株。据我们所知，这是第一次对X的遗传多样性进行描述。耳霉致病变种基于tale基因分析的稻生菌菌株
Xanthomonas oryzae pv. oryzicola causes bacterial leaf streak (BLS), a devastating disease of rice in Asia countries. X. otyzae pv. oryzicola utilizes repertoires of transcriptional activator-like effectors (TALEs) to manipulate host resistance or susceptibility; thus, TALEs can determine the outcome of BLS. In this report, we studied genetic diversity in putative tale genes of 65 X. otyzae pv. wyzico/a strains that originated from nine provinces of southern China. Genomic DNAs from the 65 strains were digested with BamHI and hybridized with an internal fragment of avrXa3, a tale gene originating from the related pathogen, X. otyzae pv. otyzae, which causes bacterial leaf blight (BLB). Southern blot analysis indicated that the strains contained a variable number (9 to 22) of avrXa3-hybridizing fragments (e.g., putative tale genes). Based on the number and size of hybridizing bands, strains were classified into 14 genotypes (designated 1 to 14), and genotypes 3 and 10 represented 29.23 and 24.64% of the total, respectively. A high molecular weight BamHI fragment (HMWB; approximate to 6.0 kb) was present in 12 of the 14 genotypes, and sequence analysis of the HMWB revealed the presence of a C-terminally truncated tale, an insertion element related to IS1403, and genes encoding phosphoglycerate mutase and endonuclease V. Primers were developed from the 6.0-kb HMWB fragment and showed potential in genotyping X. oryzae pv. oryzicola strains by polymerase chain reaction. Virulence of X. otyzae pv. oryzicola strains was assessed on 23 rice cultivars containing different resistance genes for BLB. The X. oryzae pv. oryzicola strains could be grouped into 14 pathotypes (I to XIV), and the grouping of strains was almost identical to the categories determined by genotypic analysis. In general, strains containing higher numbers of putative tale genes were more virulent on rice than strains containing fewer tales. The results also indicate that there are no gene-for-gene relationships between the tested rice lines and X. oryzae pv. oryzicola strains. To our knowledge, this is the first description of genetic diversity of X. otyzae pv. oryzicola strains based on tale gene analysis.