Exact Solution of a Model DNA-Inversion Genetic Switch with Orientational Control
Exact Solution of a Model DNA-Inversion Genetic Switch with Orientational Control
复制标题
具有方向控制的模型DNA反转遗传开关的精确解
DOI：
10.1103/physrevlett.101.118104
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发表时间：
2008
影响因子：
8.6
通讯作者：
Visco P
中科院分区：
文献类型：
--
作者：
Visco P
DNA倒位是细菌和噬菌体在表型状态之间可逆转换的重要机制。在这样的开关中，短DNA元件的方向被位点特异性重组酶翻转。我们提出了一个简单的模型，其中重组酶的生产是依赖于开关状态（定向控制）的DNA反转开关。我们的模型的灵感来自于E.杆菌我们提出了一个精确的解析解的化学主方程的模型切换，以及随机模拟。方向控制导致开关偏离泊松行为：在开状态下的时间分布显示出峰值，并且连续的翻转时间是相关的。
DNA inversion is an important mechanism by which bacteria and bacteriophage switch reversibly between phenotypic states. In such switches, the orientation of a short DNA element is flipped by a site-specific recombinase enzyme. We propose a simple model for a DNA-inversion switch in which recombinase production is dependent on the switch state (orientational control). Our model is inspired by thefimswitch inE. coli. We present an exact analytical solution of the chemical master equation for the model switch, as well as stochastic simulations. Orientational control causes the switch to deviate from Poissonian behavior: the distribution of times in the on state shows a peak and successive flip times are correlated.