Correlation of Interleukin-6 and monocyte chemotactic protein-1 levels with the crescent formation and myeloperoxidase-specific anti-neutrophil cytoplasmic antibody titer in SCG/Kj mice by treatment with anti-interleukin-6 receptor antibody or Mizoribine.
Correlation of Interleukin-6 and monocyte chemotactic protein-1 levels with the crescent formation and myeloperoxidase-specific anti-neutrophil cytoplasmic antibody titer in SCG/Kj mice by treatment with anti-interleukin-6 receptor antibody or Mizoribine.
复制标题
使用抗白细胞介素 6 受体抗体或咪唑立宾治疗 SCG/Kj 小鼠中白细胞介素 6 和单核细胞趋化蛋白 1 水平与新月体形成和髓过氧化物酶特异性抗中性粒细胞胞浆抗体滴度的相关性。
DOI：
10.1111/1348-0421.12080
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Suzuki K.
中科院分区：
文献类型：
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作者：
Nagao T;Kusunoki R;Iwamura C;Kobayashi S;Yumura W;Kameoka Y;Nakayama T;Suzuki K.
髓过氧化物酶特异性抗中性粒细胞胞浆抗体（MPO-ANCA）与快速进展性肾小球肾炎（RPGN）和肾小球新月体形成相关。使用自发发展MPO-ANCA相关RPGN的SCG/Kj小鼠分析RPGN中的致病因素。采用ELISA法测定血清可溶性IL-6 R浓度，采用生物免疫分析法测定另外23种细胞因子和趋化因子的血清浓度。通过荧光显微镜检查冷冻肾组织切片，并通过流式细胞术测定脾脏中的CD 3 +B220+T细胞亚群。IL-6和单核细胞趋化蛋白-1的浓度与新月体形成的百分比显著相关。向SCG/Kj小鼠施用抗IL-6 R抗体，该抗体在类风湿性关节炎患者中有效，以阐明IL-6在RPGN发展中的作用。给予抗IL-6 R抗体后，MPO-ANCA滴度降低，但咪唑立宾滴度未降低，咪唑立宾在川崎模型小鼠中有效。这些结果表明，IL-6介导的信号转导参与了MPO-ANCA的产生。
Myeloperoxidase‐specific anti‐neutrophil cytoplasmic antibody (MPO–ANCA) is associated with rapidly progressive glomerulonephritis (RPGN) and glomerular crescent formation. Pathogenic factors in RPGN were analyzed by using SCG/Kj mice, which spontaneously develop MPO–ANCA‐associated RPGN. The serum concentration of soluble IL‐6R was determined by using ELISA and those of another 23 cytokines and chemokines by Bio‐Plex analysis. Sections of frozen kidney tissue were examined by fluorescence microscopy and the CD3+B220+T cell subset in the spleen determined by a flow cytometry. Concentrations of IL‐6 and monocyte chemotactic protein‐1 were significantly correlated with the percentages of crescent formation. Anti‐IL‐6R antibody, which has been effective in patients with rheumatoid arthritis, was administered to SCG/Kj mice to elucidate the role of IL‐6 in the development of RPGN. MPO–ANCA titers decreased after administration of anti‐IL‐6R antibody, but not titers of mizoribine, which is effective in Kawasaki disease model mice. These results suggest that IL‐6‐mediated signaling is involved in the production of MPO–ANCA.