Involvement of gluconic acid and glucose oxidase in the pathogenicity of Penicillium expansum in apples
Involvement of gluconic acid and glucose oxidase in the pathogenicity of Penicillium expansum in apples
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DOI：
10.1094/phyto-97-3-0384
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发表时间：
2007-03-01
期刊：
影响因子：
3.2
通讯作者：
Prusky, Dov
中科院分区：
文献类型：
--
作者：
Hadas, Yoav;Goldberg, Israel;Prusky, Dov
通过调控侵染区葡萄糖酸积累量的增加或减少，分析了葡萄糖酸分泌对扩展青霉在苹果组织定殖的贡献。表达最多gox2转录物和伴随的葡萄糖氧化酶（GOX）活性以及分泌最多葡萄糖酸的扩展疫霉分离物以最快的速度引起苹果病害。在低氧浓度下生长的培养物产生的gox2转录物较少，产生的葡萄糖酸较少，导致疾病减少15%。此外，在腐烂组织的边缘处检测到显著高水平的gox 2和GOX活性的转录物强调了GOX参与腐烂组织的组织酸化。总之，这些结果强调了GOX在葡萄糖酸产生中的重要性，葡萄糖酸反过来导致宿主组织酸化。这种酸化增强了果胶溶解酶的表达，并建立了扩展青霉坏死营养发育的条件。
The contribution of gluconic acid secretion to the colonization of apple tissue by Penicillium expansum was analyzed by modulation (increase or decrease) of gluconic acid accumulation at the infection court. P. expansum isolates that express the most gox2 transcripts and concomitant glucose oxidase (GOX) activity and that secrete the most gluconic acid cause disease of apple at the fastest rate. Cultures grown under reduced oxygen concentration generated fewer gox2 transcripts, produced less gluconic acid, and led to a 15% reduction in disease. Furthermore, the detection of significantly high levels of transcripts of gox2 and GOX activity at the edge of the decaying tissue emphasize the involvement of GOX in tissue acidification of the decaying tissue. Taken together, these results emphasize the importance of GOX in the production of the gluconic acid that leads, in turn, to host tissue acidification. This acidification enhanced the expression of pectolytic enzymes and the establishment of conditions for necrotrophic development of P. expansum.