Nasal carriage of Staphylococcus aureus in healthy humans with different levels of contact with animals in Tunisia: genetic lineages, methicillin resistance, and virulence factors
Nasal carriage of Staphylococcus aureus in healthy humans with different levels of contact with animals in Tunisia: genetic lineages, methicillin resistance, and virulence factors
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DOI：
10.1007/s10096-010-1109-6
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发表时间：
2011-04-01
影响因子：
4.5
通讯作者：
Torres, C.
中科院分区：
文献类型：
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作者：
Ben Slama, K.;Gharsa, H.;Torres, C.
在突尼斯（2008-2009年）获得了423名健康人的鼻拭子，这些人显示出与动物不同程度的接触（频繁，168人;零星，94人;无接触，161人），其中99人存在其他相关风险因素。在从兽医处获取的423份样本中的1份（0.24%）中检测到耐甲氧西林金黄色葡萄球菌（MRSA）。MRSA分离株为mecA阳性，分型为ST 80-t203-SCCmecIVc-agrIII，并含有分别编码四环素、链霉素和卡那霉素抗性的泰特（K）、ant（6）-Ia和aph（3 '）-IIIa基因。该MRSA分离株还含有编码Panton-Valentine杀白细胞素的lukF/lukS毒力基因。另外54份（12.8%）鼻样本含有甲氧西林敏感的S。金黄色葡萄球菌（MSSA）和一个分离物/样品进行了表征。在55个回收的S中检测到高多样性的spa型（n = 43; 4个新的）和脉冲场凝胶电泳（PFGE）型（n = 37）。金黄色葡萄球菌抗生素耐药/检测到的耐药基因百分比如下：四环素[22%/泰特（K）-泰特（L）-泰特（M）]、红霉素[5%/msrA]、环丙沙星[14.5%]、甲氧苄啶-磺胺甲恶唑[2%/dfrA]、链霉素[11%/ant（6）-Ia]、卡那霉素[7%/aph（3 '）-IIIa]、阿米卡星[5%]和氯霉素[2%]。四个和两个分离株携带lukF/lukS和eta和/或etb基因，分别，总是在与动物接触的个人。11个分离株携带tst基因，并从与动物有不同程度接触的个体中回收。
Nasal swabs of 423 healthy humans who showed different levels of contact with animals (frequent, 168; sporadic, 94; no contact, 161) were obtained in Tunisia (2008-2009), and 99 of them presented other associated risk factors. Methicillin-resistant Staphylococcus aureus (MRSA) was detected in one of these 423 samples (0.24%), retrieved from a veterinarian. The MRSA isolate was mecA-positive, typed as ST80-t203-SCCmecIVc-agrIII, and contained tet(K), ant(6)-Ia, and aph(3')-IIIa genes encoding tetracycline, streptomycin, and kanamycin resistance, respectively. This MRSA isolate also contained the lukF/lukS virulence gene encoding Panton-Valentine leukocidin. Fifty-four (12.8%) additional nasal samples contained methicillin-susceptible S. aureus (MSSA) and one isolate/sample was characterized. A high diversity of spa types (n = 43; 4 new) and pulsed-field gel electrophoresis (PFGE) types (n = 37) was detected among the 55 recovered S. aureus strains. The percentages of antimicrobial resistance/detected resistance genes were as follows: tetracycline [22%/tet(K)-tet(L)-tet(M)], erythromycin [5%/msrA], ciprofloxacin [14.5%], trimethoprim-sulfamethoxazole [2%/dfrA], streptomycin [11%/ant(6)-Ia], kanamycin [7%/aph(3')-IIIa], amikacin [5%], and chloramphenicol [2%]. Four and two isolates carried the lukF/lukS and eta and/or etb genes, respectively, and always in individuals with contact with animals. Eleven isolates carried the tst gene and were recovered from individuals with different levels of contact with animals.