Primary structure and gene organization of human hepatitis A virus.
Primary structure and gene organization of human hepatitis A virus.
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DOI：
10.1073/pnas.82.9.2627
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发表时间：
1985-05
影响因子：
11.1
通讯作者：
R. Najarian;D. Caput;W. Gee;S. J. Potter;A. Renard;J. Merryweather;G. Nest;D. Dina
中科院分区：
文献类型：
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作者：
R. Najarian;D. Caput;W. Gee;S. J. Potter;A. Renard;J. Merryweather;G. Nest;D. Dina
对甲型肝炎病毒(HAV)的RNA基因组进行了分子克隆。用代表整个甲型肝炎病毒RNA的重组DNA克隆来确定病毒基因组的一级结构。病毒基因组的长度为7478个核苷酸。起始于核苷酸734、终止于核苷酸7415的开放阅读框编码MR 251,940的多蛋白。将甲型肝炎病毒的核苷酸序列与其他小核糖核酸病毒的核苷酸序列进行比较，未能揭示可检测到的同源性区域。然而，对甲型肝炎病毒和脊髓灰质炎病毒推定的氨基酸序列的计算机分析表明，病毒粒子蛋白3(VP3)存在较短的同源性区域，并贯穿于多蛋白的羧基末端部分。此外，还检测到与脊髓灰质炎病毒广泛的蛋白质结构同源性。
The RNA genome of human hepatitis A virus (HAV) was molecularly cloned. Recombinant DNA clones representing the entire HAV RNA were used to determine the primary structure of the viral genome. The length of the viral genome is 7478 nucleotides. An open reading frame starting at nucleotide 734 and terminating at nucleotide 7415 encodes a polyprotein of Mr 251,940. Comparison of the HAV nucleotide sequence with that of other picornaviruses has failed to reveal detectable areas of homology. However, a computer analysis of the putative amino acid sequence of HAV and poliovirus demonstrated the existence of short areas of homology in virion protein 3 (VP3) and throughout the carboxyl-terminal portion of the polyproteins. In addition, extensive protein structural homologies with poliovirus were detected.