Electrical stimulation based on chronaxie reduces atrogin-1 and MyoD gene expressions in denervated rat muscle
Electrical stimulation based on chronaxie reduces atrogin-1 and MyoD gene expressions in denervated rat muscle
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DOI：
10.1002/mus.20668
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发表时间：
2007-01-01
期刊：
影响因子：
3.4
通讯作者：
Salvini, Tania Fatima
中科院分区：
文献类型：
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作者：
Russo, Thiago Luiz;Peviani, Sabrina Messa;Salvini, Tania Fatima
去神经支配诱导骨骼肌肌纤维萎缩和骨骼肌基因表达率的改变。电刺激（ES）是一种通常用于治疗人类失神经肌肉的方法。本研究评价了基于chronaxie和rheobase的ES对Wistar大鼠失神经胫前肌myoD和atroggin -1基因表达的影响。检查五组：(1)去神经组(D)；(2) D + ES;(3)假性去神经；(4)正常(N)；(5) N+ES。用表面电极每48 h刺激20次肌肉收缩。28 D后，与D组相比，ES显著降低了D+ES中myoD和atrogin-1的表达。然而，ES并不能预防去神经支配后的肌纤维萎缩。因此，基于chronaxie值并使用表面电极将ES应用于失神经肌肉，就像通常用于人类康复一样，能够降低myoD和atrogin1基因表达，这两个基因分别与肌肉生长和萎缩有关。本研究结果为表面ES治疗失神经支配骨骼肌提供了新的信息。
Denervation induces muscle fiber atrophy and changes in the gene expression rates of skeletal muscle. Electrical stimulation (ES) is a procedure generally used to treat denervated muscles in humans. This study evaluated the effect of ES based on chronaxie and rheobase on the expression of the myoD and atrogin-1 genes in denervated tibialis anterior (TA) muscle of Wistar rats. Five groups were examined: (1) denervated (D); (2) D+ES; (3) sham denervation; (4) normal (N); and (5) N+ES. Twenty muscle contractions were stimulated every 48 h using surface electrodes. After 28 days, ES significantly decreased the expression of myoD and atrogin-1 in D+ES compared to the D group. However, ES did not prevent muscle-fiber atrophy after denervation. Thus, ES based on chronaxie values and applied to denervated muscles using surface electrodes, as normally used in human rehabilitation, was able to reduce the myoD and atrogin-1 gene expressions, which are related to muscular growth and atrophy, respectively. The results of this study provide new information for the treatment of denervated skeletal muscle using surface ES.