Yersinia high pathogenicity island genes modify the Escherichia coli primary metabolome independently of siderophore production.
Yersinia high pathogenicity island genes modify the Escherichia coli primary metabolome independently of siderophore production.
复制标题
DOI：
10.1021/pr200756n
复制
发表时间：
2011-12-02
影响因子：
4.4
通讯作者：
Henderson, Jeffrey P.
中科院分区：
文献类型：
--
作者：
Lv, Haitao;Henderson, Jeffrey P.
关键词：
细菌铁载体可能通过摄取铁来增强致病性，但其表达被认为会产生巨大的代谢成本。在此，我们描述了一种代谢组学 - 遗传学联合方法，以确定影响与毒力相关的铁载体耶尔森菌素的突变如何影响大肠杆菌的初级代谢组。与预期相反，我们未发现耶尔森菌素的生物合成与一致的代谢组变化相对应。相反，我们发现靶向缺失ybtU或ybtA（在调节耶尔森菌素表达中作用相似的不同基因），在基本培养基中生长时与精氨酸途径代谢物的特定变化相关。与ybtU和ybtA突变体以及缺乏耶尔森菌素相关基因的K12菌株MG1655相比，这种相互作用与尿路致病性大肠杆菌UTI89模型中的高精氨酸水平相关。由于精氨酸不是耶尔森菌素生物合成的直接底物，这些发现表明与毒力相关的次级代谢物系统可能独立于底物消耗而影响细菌的初级代谢。
Bacterial siderophores may enhance pathogenicity by scavenging iron but their expression has been proposed to exert a substantial metabolic cost. Here we describe a combined metabolomic-genetic approach to determine how mutations affecting the virulence-associated siderophore yersiniabactin affect the Escherichia coli primary metabolome. Contrary to expectations, we did not find yersiniabactin biosynthesis to correspond to consistent metabolomic shifts. Instead, we found that targeted deletion of ybtU or ybtA, dissimilar genes with similar roles in regulating yersiniabactin expression, were associated with a specific shift in arginine pathway metabolites during growth in minimal media. This interaction was associated with high arginine levels in the model uropathogen Escherichia coli UTI89 compared to its ybtU and ybtA mutants and the K12 strain MG1655, which lacks yersiniabactin-associated genes. Because arginine is not a direct yersiniabactin biosynthetic substrate, these findings show that virulence-associated secondary metabolite systems may shape bacterial primary metabolism independently of substrate consumption.
登录
查看更多内容
影响因子：
6.7
作者：
Henderson, Jeffrey P.;Crowley, Jan R.;Pinkner, Jerome S.;Walker, Jennifer N.;Tsukayama, Pablo;Stamm, Walter E.;Hooton, Thomas M.;Hultgren, Scott J.
通讯作者：
Hultgren, Scott J.
影响因子：
10.4
作者：
Tong W;Cao X;Harris S;Sun H;Fang H;Fuscoe J;Harris A;Hong H;Xie Q;Perkins R;Shi L;Casciano D
通讯作者：
Casciano D
影响因子：
--
作者：
de Carvalho, Luiz Pedro S.;Fischer, Steven M.;Rhee, Kyu Y.
通讯作者：
Rhee, Kyu Y.
影响因子：
--
作者：
Murphy KC;Campellone KG
通讯作者：
Campellone KG
影响因子：
3.2
作者：
Amador-Noguez, Daniel;Feng, Xiao-Jiang;Rabinowitz, Joshua D.
通讯作者：
Rabinowitz, Joshua D.