Social Motility Assays of Flavobacterium johnsoniae

Social Motility Assays of Flavobacterium johnsoniae
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约氏黄杆菌的社会运动测定

DOI:
10.1007/978-1-0716-3060-0_23
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发表时间:
2023
期刊:
Methods Molecular Biology
影响因子:
--
通讯作者:
Keiko Sato
Keiko Sato
中科院分区:
--
文献类型:
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作者:
Chikara Sato;Keiko Sato

文献摘要

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约翰黄杆菌细胞通过滑动运动在固体表面快速移动。 F的集体迁徙约翰森氏菌表面会导致形成蔓延的菌落。集落扩散受到细胞表面粘附素成分以及琼脂和葡萄糖浓度的影响。例如,在营养贫乏的琼脂培养基上,形成薄膜状、圆形散布的菌落。 johnsoniae表现出至少两种类型的迁移:导致同心集落的小细胞簇运动和导致树突集落的单个细胞运动。本章介绍了观察菌落形态的方法。
Flavobacterium johnsoniaecells move rapidly over solid surfaces by gliding motility. The collective migration ofF. johnsoniaeon the surfaces results in the formation of spreading colonies. Colony spreading is influenced by adhesin components on the cell surface and the concentrations of agar and glucose. For example, on nutrient-poor agar media, film-like, round spreading colonies are formed.F. johnsoniaedisplays at least two types of migration: small cell cluster movements leading to concentric colonies and individual cell movements leading to dendritic colonies. The methods for observing colony morphology are described in this chapter.