Coordinated regulation of the genes participating in starch biosynthesis by the rice Floury-2 locus
Coordinated regulation of the genes participating in starch biosynthesis by the rice Floury-2 locus
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DOI：
10.1104/pp.110.1.89
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发表时间：
1996-01-01
期刊：
影响因子：
7.4
通讯作者：
Baba, T
中科院分区：
文献类型：
--
作者：
Kawasaki, T;Mizuno, K;Baba, T
水稻（Oryza sativa L.）的隐性floury-2（flo-2）位点，其位于染色体4上，导致开花后10天未成熟种子中编码分支酶RBE 1同种型的基因的表达强烈降低。RBE 1基因定位于水稻6号染色体上，表明野生型Floury-2（Flo-2）基因调控RBE 1基因的反式表达，但在Flo-2种子中，RBE 1基因的表达降低，其中包括另一种分支酶RBE 3和颗粒结合型淀粉合成酶。尽管在flo-2突变体的未成熟种子中RBE 1基因的表达水平较低，但RBE 1基因在野生型和flo-2突变体的叶片中表达相同。因此，这些结果意味着Flo-2基因可能以发育种子特异性的方式共同调节参与淀粉合成的一些基因的表达。
The recessive floury-2 (flo-2) locus of rice (Oryza sativa L.), which is located on chromosome 4, causes a strong reduction in expression of the gene encoding an isoform of branching enzyme RBE1 in immature seeds 10 d after flowering. Mapping of the RBE1 gene demonstrated the localization on rice chromosome 6, suggesting that the wild-type Floury-2 (Flo-2) gene regulates RBE1 gene expression in trans. However, reduced expression of the genes encoding some other starch-synthesizing enzymes, including another isoform of branching enzyme RBE3 and granule-bound starch synthase, was also found in the flo-2 seeds. In spite of the low level of RBE1 gene expression in the immature seeds of the flo-2 mutants, the RBE1 gene was equally expressed in the leaves of the wild type and flo-2 mutants. Thus, these results imply that the Flo-2 gene may co-regulate expression of some of the genes participating in starch synthesis possibly in a developing seed-specific manner.