A histone H4 gene prevents drought-induced bolting in Chinese cabbage by attenuating the expression of flowering genes
A histone H4 gene prevents drought-induced bolting in Chinese cabbage by attenuating the expression of flowering genes
复制标题
组蛋白 H4 基因通过减弱开花基因的表达来防止大白菜干旱诱导的抽苔
DOI：
10.1093/jxb/eraa452
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发表时间：
2020
影响因子：
6.9
通讯作者：
Fenglan Zhang
中科院分区：
文献类型：
--
作者：
Xiaoyun Xin;Tongbing Su;Peirong Li;Weihong Wang;Xiuyun Zhao;Yangjun Yu;Deshuang Zhang;Shuancang Yu;Fenglan Zhang
开花是大白菜的一个重要性状，因为早花会降低收获产品的产量和质量。干旱或其他环境条件引起的水分亏缺会导致早花。抗旱性涉及转录、激素信号和染色质修饰的全球重新编程。我们发现组蛋白H4蛋白BrHIS4.A04与同源结构域蛋白BrVIN3.1物理上相互作用，BrVIN3.1是在晚抽蕾大白菜驯化过程中选择的。BrHIS4.A04的过度表达导致了正常生长条件下的提前开花，但阻止了干旱条件下进一步的提前抽苔。我们发现在干旱条件下，在BrHIS4.A04过度表达(BrHIS4.A04OE)的植物中，关键的脱落酸(ABA)信号转导基因和光周期开花基因的表达都减弱了。此外，在BrHIS4.A04OE型植株中，这些基因座上H4-乙酰化的相对变化较小。我们认为BrHIS4.A04通过ABA信号通路减弱干旱条件下光周期开花基因的表达，从而防止早熟抽苔。由于BrHIS4.A04植物在实验室诱导的干旱条件下没有表现出与营养或生殖发育相关的表型，我们的发现有助于通过基因工程微调作物的开花时间而不会造成任何生长损失，尽管在田间干旱条件下需要更多的数据。
Flowering is an important trait in Chinese cabbage, because premature flowering reduces yield and quality of the harvested products. Water deficit, caused by drought or other environmental conditions, induces early flowering. Drought resistance involves global reprogramming of transcription, hormone signaling, and chromatin modification. We show that a histone H4 protein, BrHIS4.A04, physically interacts with a homeodomain protein BrVIN3.1, which was selected during the domestication of late-bolting Chinese cabbage. Over-expression ofBrHIS4.A04resulted in premature flowering under normal growth conditions, but prevented further premature bolting in response to drought. We show that the expression of key abscisic acid (ABA) signaling genes, and also photoperiodic flowering genes was attenuated inBrHIS4.A04-overexpressing (BrHIS4.A04OE) plants under drought conditions. Furthermore, the relative change in H4-acetylation at these gene loci was reduced inBrHIS4.A04OEplants. We suggest that BrHIS4.A04 prevents premature bolting by attenuating the expression of photoperiodic flowering genes under drought conditions, through the ABA signaling pathway. SinceBrHIS4.A04OEplants displayed no phenotype related to vegetative or reproductive development under laboratory-induced drought conditions, our findings contribute to the potential fine-tuning of flowering time in crops through genetic engineering without any growth penalty, although more data are necessary under field drought conditions.