eSex differences in constitutive mRNA levels of CYP2B22, CYP2C33, CYP2C49, CYP3A22, CYP3A29 and CYP3A46 in the pig liver: Comparison between Meishan and Landrace pigs
eSex differences in constitutive mRNA levels of CYP2B22, CYP2C33, CYP2C49, CYP3A22, CYP3A29 and CYP3A46 in the pig liver: Comparison between Meishan and Landrace pigs
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DOI：
10.1016/j.dmpk.2016.02.001
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发表时间：
2016-06-01
影响因子：
2.1
通讯作者：
Degawa, Masakuni
中科院分区：
文献类型：
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作者：
Kojima, Misaki;Degawa, Masakuni
在 5 个月大的梅山猪、长白猪及其杂交 F-1（LM 和 ML）猪中检查了细胞色素 P450 (CYP) 同工型（CYP2B22、CYP2C33、CYP2C49、CYP3A22、CYP3A29 和 CYP3A46）肝脏 mRNA 水平的品种和性别差异。雄性梅山猪、LM猪和ML猪的血清睾酮水平比长白猪高2.5-3.5倍。 CYP3A46 mRNA 仅在长白猪、LM 猪和 ML 猪中进行了品种特异性检测。仅在眉山猪、LM 猪和 ML 猪中，观察到 CYP2B22、CYP2C33、CYP2C49 和 CYP3A29 mRNA 的雄性表达为主； CYP3A22 mRNA 表达显示相反的模式。在 LM 和 ML 猪中也观察到 CYP3A46 的雄性主导 mRNA 表达。当雄性被阉割后，梅山猪 CYP mRNA 水平的性别差异消失，并通过给阉割的雄性施用丙酸睾酮（TP）来恢复。在长白猪中，对阉割的雄性猪和完整的雌性猪施用 TP 显着增加了 CYP2B22、CYP2C33 和 CYP3A46 mRNA 的水平。未成熟（1 个月大）猪的 CYP mRNA 表达没有表现出品种或性别差异。结果表明，雄激素是几种 CYP 性别相关表达的重要决定因素，并表明性别相关表达的品种差异可能是由血清雄激素水平的差异和其他遗传性状引起的。 (C) 2016 年日本异生素研究会。由爱思唯尔有限公司出版。保留所有权利。
Breed and sex differences in hepatic mRNA levels of cytochrome P450 (CYP) isoforms (CYP2B22, CYP2C33, CYP2C49, CYP3A22, CYP3A29 and CYP3A46) were examined in 5-month-old Meishan, Landrace, and their crossbred F-1 (LM and ML) pigs. Serum testosterone levels in male Meishan, LM, and ML pigs were 2.5-3.5-fold higher than in Landrace pigs. CYP3A46 mRNA was breed-specifically detected only in Landrace, LM, and ML pigs. In Meishan, LM, and ML pigs only, male- predominant expressions of CYP2B22, CYP2C33, CYP2C49 and CYP3A29 mRNAs were observed; CYP3A22 mRNA expression showed the opposite pattern. Male-dominant mRNA expression was also observed in LM and ML pigs for CYP3A46. The sex differences in CYP mRNA levels in Meishan pigs disappeared when males were castrated and were restored by testosterone propionate (TP) administration to the castrated males. In Landrace pigs, TP administration to castrated males and intact females significantly increased the levels of CYP2B22, CYP2C33, and CYP3A46 mRNAs. Immature (1-month-old) pigs showed no breed or sex differences in CYP mRNA expressions. The results demonstrated that androgen is an important determinant of sex-associated expression of several CYPs and suggested that breed differences in sex-associated expression could be caused by differences in serum androgen level and by other genetic traits. (C) 2016 The Japanese Society for the Study of Xenobiotics. Published by Elsevier Ltd. All rights reserved.