IMPROVED STAINING OF PROTEINS IN POLYACRYLAMIDE GELS INCLUDING ISOELECTRIC-FOCUSING GELS WITH CLEAR BACKGROUND AT NANOGRAM SENSITIVITY USING COOMASSIE BRILLIANT BLUE G-250 AND R-250
IMPROVED STAINING OF PROTEINS IN POLYACRYLAMIDE GELS INCLUDING ISOELECTRIC-FOCUSING GELS WITH CLEAR BACKGROUND AT NANOGRAM SENSITIVITY USING COOMASSIE BRILLIANT BLUE G-250 AND R-250
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DOI：
10.1002/elps.1150090603
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发表时间：
1988-06-01
期刊：
影响因子：
2.9
通讯作者：
EHRHARDT, W
中科院分区：
文献类型：
--
作者：
NEUHOFF, V;AROLD, N;EHRHARDT, W
介绍了一种利用考马斯亮蓝G-250和R-250的胶体性质，在聚丙烯酰胺凝胶中分离后对蛋白质进行染色的改进方法。新方法是在上述染色溶液中加入20%v/v甲醇和更高浓度的硫酸铵[1]。该方法结合了染色时间短、灵敏度高、背景清晰无需脱色、染色后分步染色、固定稳定等优点。该方法已用于十二烷基硫酸钠电泳法和载体两性阳离子产生的pH梯度等电聚焦后的聚丙烯酰胺凝胶染色。
An improved procedure for staining of proteins following separation in polyacrylamide gels is described which utilizes the colloidal properties of Coomassie brilliant Blue G-250 and R-250. The new method is based on addition of 20% v/v methanol and higher concentrations of ammonium sulfate to the staining solution previously described [1]. The method combines the advantage of much shorter staining time with high sensitivity, a clear background not requiring destainig, stepwise staining, and stable fixation after staining. The method has been applied to staining of polyacrylamide gels after sodium dodecyl sulfate-electrophoresis and isoelectric focusing in carrier ampholyte-generated pH gradients.