Regulation of stearoyl-CoA desaturase 1 mRNA stability by polyunsaturated fatty acids in 3T3-L1 adipocytes
Regulation of stearoyl-CoA desaturase 1 mRNA stability by polyunsaturated fatty acids in 3T3-L1 adipocytes
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DOI：
10.1074/jbc.271.47.29854
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发表时间：
1996-11-22
影响因子：
4.8
通讯作者：
Ntambi, JM
中科院分区：
文献类型：
--
作者：
Sessler, AM;Kaur, N;Ntambi, JM
在完全分化的 3T3-L1 脂肪细胞中研究了花生四烯酸 (20:4, n-6) 和其他脂肪酸对硬脂酰辅酶 A 去饱和酶基因 1 表达的影响。用花生四烯酸处理3T3-L1脂肪细胞会导致硬脂酰辅酶A去饱和酶（Scd）酶活性和scd1 mRNA降低，花生四烯酸不会改变scd1基因的转录，而scd1 mRNA的半衰期从25.1小时缩短至8.5小时。环氧合酶、脂氧合酶或细胞色素 P-450 环氧化酶抑制剂不能逆转花生四烯酸引起的抑制，表明类二十烷酸的合成对于抑制 scd1 mRNA 表达不是必需的。用亚油酸（18:2，n-6）和亚麻酸（18:3，n-3）处理脂肪细胞也导致 scd1 mRNA 积累的抑制。相比之下，油酸（18:1，n-9）和硬脂酸（18:0）对 scd1 mRNA 水平没有影响。综上所述，这些结果表明多不饱和脂肪酸通过降低 scd1 mRNA 的稳定性来抑制成熟脂肪细胞中 scd1 基因的表达。
The effects of arachidonic acid (20:4, n-6) and other fatty acids on the expression of stearoyl-CoA desaturase gene 1 were investigated in fully differentiated 3T3-L1 adipocytes. Treatment of 3T3-L1 adipocytes with arachidonic acid resulted in a decrease in stearoyl-CoA desaturase (Scd) enzyme activity and scd1 mRNA, Arachidonic acid did not alter the transcription of the scd1 gene, whereas the half-life of the scd1 mRNA was reduced from 25.1 to 8.5 h, Blocking the conversion of arachidonic acid to eicosanoids by pretreatment of the cells with cyclooxygenase, lipoxygenase, or cytochrome P-450 epoxygenase inhibitors did not reverse the inhibition caused by arachidonic acid, indicating that eicosanoid synthesis is not necessary for the repression of scd1 mRNA expression. Treatment of adipocytes with linoleic (18:2, n-6) and linolenic (18:3, n-3) acids also resulted in inhibition of scd1 mRNA accumulation. By contrast, oleic acid (18:1, n-9) and stearic acid (18:0) had no effect on scd1 mRNA levels. Taken together, these results suggest that polyunsaturated fatty acids repress the expression of the scd1 gene in mature adipocytes by reducing the stability of scd1 mRNA.