Molecular cloning of Bombyx cerebral opsin (Boceropsin) and cellular localization of its expression in the silkworm brain.-猫眼课题宝

家蚕脑视蛋白（Boceropsin）的分子克隆及其在家蚕大脑中表达的细胞定位。

DOI：

发表时间：

2001

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Biochemical and Biophysical Research Communications - BBRC

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作者：

I. Shimizu;Y. Yamakawa;Y. Shimazaki;T. Iwasa

我们从家蚕幼虫的大脑中克隆了一种新的视蛋白cDNA，这种视蛋白的光周期感受器被认为存在于头侧中枢神经系统（CNS）中。推导出的氨基酸序列由381个氨基酸组成，包含昆虫视色素中高度保守的氨基酸残基。该视蛋白属于昆虫视蛋白的长波长感受器群，与鞘蛾的绿色视感受器同源度最高（84%）。我们将这种家蚕脑视蛋白命名为Boceropsin。Southern blotting实验表明Boceropsin基因存在于单个拷贝中，RT-PCR分析显示Boceropsin mRNA在幼虫大脑中表达，而在食管下神经节（Sg）和胸神经节（Tg）中不表达。免疫组化分析表明，Boceropsin蛋白双侧存在于家蚕幼虫脑部的某些特定细胞中。这是首次报道视蛋白蛋白在昆虫中枢神经系统中表达。讨论了Boceropsin在家蚕中作为光周期受体色素的可能性。

We have cloned a cDNA for a novel opsin from the larval brain of the silkworm Bombyx mori in which the photoperiodic photoreceptor had been supposed to reside in the cephalic central nervous system (CNS). Its deduced amino acid sequence was composed of 381 amino acids and included amino acid residues highly conserved in insect visual pigments. This opsin belonged to the long wavelength photoreceptor group of insect opsins and showed the greatest degree of homology (84%) with the green visual photoreceptor in the sphingid moth. We have designated this Bombyx cerebral opsin as Boceropsin. Southern blotting experiments indicated that the Boceropsin gene is present in a single copy, and RT-PCR analysis revealed that Boceropsin mRNA is expressed in the larval brain but not in the subesophageal ganglion (Sg) or thoracic ganglion (Tg). Immunohistochemical analyses demonstrated that Boceropsin protein is present bilaterally in some defined cells localized in the brain of Bombyx larvae. This is the first report of expression of an opsin-based protein in CNS of an insect. The possibility that the Boceropsin functions as the photoperiodic receptive pigment in the silkworm is also discussed.

In vitro reprogramming of the photoperiodic clock in an insect brain-retrocerebral complex.