Activated natural killer cell tumor retention and cytokine production in colon tumor using a tissue-isolated model.
Activated natural killer cell tumor retention and cytokine production in colon tumor using a tissue-isolated model.
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使用组织隔离模型激活结肠肿瘤中自然杀伤细胞的肿瘤保留和细胞因子的产生。
DOI：
10.1006/jsre.1998.5521
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Posner,MC
中科院分区：
文献类型：
--
作者：
RibeiroJr,U;Whiteside,TL;Basse,PH;Safatle-Ribeiro,AV;Huneke,CE;Posner,MC
BackgroundAdoptively transferred activated natural killer (A-NK) cells are capable of selectively infiltrating solid tumors, but only at low efficiency when administered systemically. It is unclear if human A-NK cells can be retained in tumor tissue and, if so, what is their action. We investigated intratumor A-NK cell retention andin situcytokine production, using an xenogeneicex vivotissue-isolated tumor model, which permits direct intraarterial infusion.Materials and MethodsHuman colon adenocarcinoma (HT-29) was implanted in the ovarian fat pad of nude rats. The tumors were perfusedex vivo25 to 30 days postimplant with a known number of cells, and the effluent was collected over time. The number of human A-NK cells and cell surface antigen expression of cells infused and exiting the tumor were calculated, using cell counts and flow cytometry, respectively. Frozen sections were stained with Giemsa and also immunostained for the presence of interleukin-2, -4, and -10, tumor necrosis factor α (TNF-α), and interferon.ResultsSix perfusions with 8 × 106A-NK cells were performed. The mean number of infused A-NK cells that remained in the tumor at the completion of perfusion was 4.74 × 106(59.2%). No differences were noted in cellular phenotype between the infused cells and the cells exiting the tumor: expression of the markers CD45 (97.5% vs 94.5%), CD14 (0 vs 0), CD3 (3.83% vs 2.83%), and CD56 (86% vs 83%) was unchanged,P> 0.05. Microscopic examination of tumor sections showed tumor surrounded by A-NK cells, with some tumor nests infiltrated by A-NK cells.In situimmunopositivity for interleukin-2 (2/6), interleukin-4 (3/6), interleukin-10 (2/6), and TNF-α (2/6) specimens was observed. Immunostaining for interferon-γ was negative.ConclusionsThe retention of A-NK cells in the transplanted human colon tumor tissue was found to be efficient (59.2 %) in this model. Although perfusion time was limited, A-NK cells were able to infiltrate the tumor and initiate cytokine production.
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过继转移贴壁 LAK 细胞的组织分布：给药途径的作用。
DOI：
--
发表时间：
1992
期刊：
Natural immunity
影响因子：
--
作者：
Basse,PH;Herberman,RB;Hokland,ME;Goldfarb,RH
通讯作者：
Goldfarb,RH
影响因子：
11.2
作者：
N. Z. Wu;B. Klitzman;R. Dodge;M. Dewhirst
通讯作者：
N. Z. Wu;B. Klitzman;R. Dodge;M. Dewhirst
影响因子：
11.2
作者：
J. R. Less;T. Skalak;E. Sevick;R. Jain
通讯作者：
J. R. Less;T. Skalak;E. Sevick;R. Jain
人类 NK 细胞产生 IL-5，并通过 IL-4、IL-10 和 IL-12 调节 IL-5 分泌。
影响因子：
4.4
作者：
H. Warren;B. Kinnear;J. Phillips;L. Lanier
通讯作者：
L. Lanier
实体组织中人 IL-2 激活的自然杀伤细胞亚群的抗肿瘤活性。
影响因子：
4.4
作者：
N. Vujanović;S. Yasumura;H. Hirabayashi;Wen;Simon C Watkins;R. Herberman;T. Whiteside
通讯作者：
T. Whiteside