The role of the chi1 gene from the endophytic bacteria Serratia proteamaculans 336x in the biological control of wheat take-all.
The role of the chi1 gene from the endophytic bacteria Serratia proteamaculans 336x in the biological control of wheat take-all.
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DOI：
10.1139/cjm-2014-0212
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发表时间：
2014-06
影响因子：
2.8
通讯作者：
Miao Wang;Yuwan Xing;Junfang Wang;Yubin Xu;Gang Wang
中科院分区：
文献类型：
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作者：
Miao Wang;Yuwan Xing;Junfang Wang;Yubin Xu;Gang Wang
小麦全蚀病是小麦最重要的根系病害，是由小麦芽孢杆菌引起的小麦全蚀病，在世界范围内造成严重的产量损失。利用微生物作为生物制剂来控制该病是很重要的，因为目前还没有抗病品种或有效的化学杀菌剂。在这项研究中，我们测试了内生细菌Serratia proteamaculans 336x产生的几丁质酶对小麦全蚀病的生物防治能力。克隆了几丁质酶基因chi1，并在大肠杆菌中进行了异源表达。重组蛋白具有几丁质酶活性和体外抗小麦革粒菌活性。通过同源重组在框内缺失chi1基因，chi1缺失突变体缺乏几丁质酶活性，生物防治效果降低42.5%。chi1基因对Δchi1突变株的补充使几丁质酶活性和生物防治效果部分恢复。这些结果支持了Chi1蛋白在蛋白链球菌336x对小麦全蚀病的生物防治过程中的作用。
Take-all, a disease caused by the fungus Gaeumannomyces graminis var. tritici, is the most important root disease of wheat and causes severe yield losses worldwide. Using microorganisms as biological agents to control the disease is important because no resistant cultivars or effective chemical fungicides are available. In this study, we tested the biological control capability of a chitinase produced by the endophytic bacterium Serratia proteamaculans 336x against wheat take-all. The chitinase gene chi1 of S. proteamaculans 336x was cloned and heterologously expressed in Escherichia coli. The recombinant protein exhibited chitinase activity and in vitro antifungal activity against G. graminis var. tritici. With in-frame deletion of the chi1 gene by homologous recombination, the chi1-deleted mutant was devoid of chitinase activity and the biocontrol efficacy was reduced by 42.5%. The complementation of the Δchi1 mutant strain by the chi1 gene resulted in the partial restoration of the chitinase activity and biocontrol efficacy. These results support a role for the Chi1 protein in the biocontrol process of S. proteamaculans 336x against wheat take-all.