Gene expression of tumour necrosis factor and insulin signalling-related factors in subcutaneous adipose tissue during the dry period and in early lactation in dairy cows.
Gene expression of tumour necrosis factor and insulin signalling-related factors in subcutaneous adipose tissue during the dry period and in early lactation in dairy cows.
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DOI：
10.1111/j.1439-0396.2010.01005.x
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发表时间：
2010-10
影响因子：
2.7
通讯作者：
H. Sadri;H. Sadri;R. Bruckmaier;H. R. Rahmani;G. Ghorbani;I. Morel;H. V. Dorland
中科院分区：
文献类型：
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作者：
H. Sadri;H. Sadri;R. Bruckmaier;H. R. Rahmani;G. Ghorbani;I. Morel;H. V. Dorland
脂肪因子的基因表达，这可能是胰岛素敏感性的基础机制的一部分，在奶牛分娩前后进行了研究。在产前（a. p.）第8周从27头奶牛中采集皮下脂肪活组织检查和血液样品，产后第1天（p. p.）在脂肪组织样品中，通过实时逆转录聚合酶链反应定量肿瘤坏死因子α（TNFα）、胰岛素非依赖性葡萄糖转运蛋白（GLUT 1）、胰岛素应答性葡萄糖转运蛋白（GLUT 4）、胰岛素受体、胰岛素受体底物1（IRS 1）、胰岛素受体底物2（IRS 2）、磷脂酰肌醇-3激酶的调节亚基（p85）和磷脂酰肌醇-3激酶的催化亚基。测定血浆中葡萄糖、β-羟基丁酸、非酯化脂肪酸（NEFA）和胰岛素的浓度。血浆参数遵循奶牛中通常观察到的模式。观察到基因表达变化，但TNFα浓度无变化，这可能表明其局部参与脂肪组织的分解代谢适应。GLUT 4和GLUT 1 mRNA丰度的变化可能反映了它们参与降低胰岛素敏感性和在泌乳早期保留葡萄糖用于牛奶合成。IRS 1、IRS 2和p85的基因表达随时间不变可能意味着它们在胰岛素敏感性动力学方面缺乏参与。或者，它可能表明这些因子的转录后修饰起作用，并可能隐藏了参与。
Gene expression of adipose factors, which may be part of the mechanisms that underlie insulin sensitivity, were studied in dairy cows around parturition. Subcutaneous fat biopsies and blood samples were taken from 27 dairy cows in week 8 antepartum (a.p.), on day 1 postpartum (p.p.) and in week 5 p.p. In the adipose tissue samples, mRNA was quantified by real-time reverse transcription polymerase chain reaction for tumour necrosis factor alpha (TNFα), insulin-independent glucose transporter (GLUT1), insulin-responsive glucose transporter (GLUT4), insulin receptor, insulin receptor substrate 1 (IRS1), insulin receptor substrate 2 (IRS2), regulatory subunit of phosphatidylinositol-3 kinase (p85) and catalytic subunit of phosphatidylinositol-3 kinase. Blood plasma was assayed for concentrations of glucose, β-hydroxybutyric acid, non-esterified fatty acids (NEFA) and insulin. Plasma parameters followed a pattern typically observed in dairy cows. Gene expression changes were observed, but there were no changes in TNFα concentrations, which may indicate its local involvement in catabolic adaptation of adipose tissue. Changes in GLUT4 and GLUT1 mRNA abundance may reflect their involvement in reduced insulin sensitivity and in sparing glucose for milk synthesis in early lactation. Unchanged gene expression of IRS1, IRS2 and p85 over time may imply a lack of their involvement in terms of insulin sensitivity dynamics. Alternatively, it may indicate that post-transcriptional modifications of these factors came into play and may have concealed an involvement.