New immobilisation method for oligonucleotides on electrodes enables highly-sensitive, electrochemical label-free gene sensing
New immobilisation method for oligonucleotides on electrodes enables highly-sensitive, electrochemical label-free gene sensing
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DOI：
10.1016/j.bios.2017.05.049
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发表时间：
2017-11-15
影响因子：
12.6
通讯作者：
Travas-Sejdic, Jadranka
中科院分区：
文献类型：
--
作者：
Aydemir, Nihan;Chan, Eddie;Travas-Sejdic, Jadranka
我们提出了一个通用的和简便的程序，用于固定的生物探针分子的电化学传感元件。我们消除了冗长的准备程序，直接功能化的电极表面的预附着探针分子的羧酸轴承三聚体的吡咯亚苯基或噻吩亚苯基。我们证明这些缀合物可以在低电位下电沉积形成纳米级多孔电活性导电聚合物膜，暴露生物探针并保留结合活性和特异性，这里以DNA传感器为例。Fe（CN）（6）（3-/4-）修饰电极的电化学反应阻抗对溶液中存在的靶DNA序列显示出显著的（低至aM）检测灵敏度。对非互补靶序列的交叉敏感性很小，并且容易制备多靶阵列。不需要标记探针或靶寡核苷酸。
We present a versatile and facile procedure for the immobilisation of bioprobe molecules to an electrochemical sensing element. We eliminate lengthy preparation procedures for direct functionalisation of electrode surfaces by pre-attaching probe molecules to carboxylic acid bearing termonomers of pyrrole phenylenes or thiophene phenylenes. We demonstrate that these conjugates can be electrodeposited at low potentials to form nano-scale porous, electroactive conducting polymer films, exposing the bioprobe and retaining activity and specificity for binding, exemplified here with DNA sensors. The electrochemical reaction impedance for Fe(CN)(6)(3-/4-) on oligonucleotide-modified electrodes showed remarkable (down to aM) detection sensitivity for target DNA sequences present in solution. Cross-sensitivity to non-complementary target sequences is small and multi target arrays are easily made. There is no need for labelling of either probe or target oligonucleotide.