Induction of Vasoactive Substances Differs in LPS-Induced and TF-Induced DIC Models in Rats
Induction of Vasoactive Substances Differs in LPS-Induced and TF-Induced DIC Models in Rats
复制标题
LPS 诱导和 TF 诱导的大鼠 DIC 模型中血管活性物质的诱导存在差异
DOI：
10.1055/s-0037-1613272
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发表时间：
2002
影响因子：
6.7
通讯作者：
S. Nakao
中科院分区：
文献类型：
--
作者：
H. Asakura;Mariko Okudaira;Tomotaka Yoshida;Y. Ontachi;M. Yamazaki;E. Morishita;K. Miyamoto;S. Nakao
我们使用两种类型的弥散性血管内凝血（DIC）模型研究了两种血管活性物质一氧化氮（NO）和内皮素（ET）在DIC病理生理学中的作用。通过尾静脉持续输注0.1、1、10或50 mg/kg脂多糖（LPS）或3.75 U/kg促凝血酶原激酶（TF）4 h诱导实验性DIC。在LPS诱导的DIC大鼠模型中，输注0.1 mg/kg或更高的LPS后，血浆NOX（NO代谢产物）和ET水平均显著升高。相反，虽然观察到血浆NOX水平的显著增加，但在TF诱导的DIC大鼠模型中仅观察到血浆ET水平的轻微增加。在TF诱导的和LPS（50 mg/dl）诱导的DIC模型中未观察到血小板或凝血酶-ATIII复合物的血浆水平的显著差异。然而，相对于LPS诱导的模型，TF诱导的模型中血浆NOX水平显著升高（p <0.01）。相反，LPS诱导DIC后血浆ET水平显著高于TF诱导DIC后血浆ET水平（p <0.01）。血管收缩，以及抑制纤溶活性，可能是导致严重的器官功能障碍的LPS诱导的DIC大鼠模型的其他因素。此外，血管舒张，以及增强的纤溶活性，可能有助于防止大鼠严重的器官功能障碍，在TF诱导的DIC模型。我们的研究结果表明，血管活性物质的调节剂，应检查在DIC的治疗。
Summary We have investigated the role of two vasoactive substances, nitric oxide (NO) and endothelin (ET), in the pathophysiology of disseminated intravascular coagulation (DIC), using two types of DIC models. Experimental DIC was induced by sustained infusion of 0.1, 1, 10, or 50 mg/kg lipopolysaccharide (LPS), or 3.75 U/kg thromboplastin (TF), for 4 h via the rat tail vein. Plasma levels of both NOX (metabolites of NO) and ET were significantly increased following infusion of 0.1 mg/kg or greater of LPS in the LPS-induced DIC rat model. In contrast, although a marked increase in the plasma levels of NOX was observed, only a slight increase in plasma ET levels was seen in the TF-induced DIC rat model. No significant differences in the plasma levels of platelets or thrombin-ATIII complex were observed among the TF-induced and LPS (50 mg/dl)-induced DIC models. However, plasma NOX levels rose significantly higher in the TF-induced model, relative to the LPS-induced model (p <0.01). Conversely, plasma ET levels were significantly greater after LPS-induction, compared to TF-induction, of DIC (p <0.01). Vasoconstriction, as well as depressed fibrinolytic activity, may be additional factors leading to severe organ dysfunction in the LPS-induced DIC rat model. Moreover, vasodilatation, as well as enhanced fibrinolytic activity, may help to prevent rats from severe organ dysfunction in the TF-induced DIC model. Our results suggest that modulator of vasoactive substances should be examined in the treatment of DIC.
影响因子：
37.8
作者：
Yinke Yang;J. Loscalzo
通讯作者：
Yinke Yang;J. Loscalzo