Quantification of the effect of substrate concentration on the conjugal transfer rate of the TOL plasmid in short‐term batch mating experiments
Quantification of the effect of substrate concentration on the conjugal transfer rate of the TOL plasmid in short‐term batch mating experiments
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短期批量交配实验中底物浓度对 TOL 质粒接合转移率影响的定量
DOI：
10.1111/j.1472-765x.1995.tb01033.x
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发表时间：
1995
影响因子：
2.4
通讯作者：
D. Stahl
中科院分区：
文献类型：
--
作者：
B. F. Smets;B. Rittmann;D. Stahl
以恶臭假单胞菌PAW 1（TOL）作为供体，铜绿假单胞菌PAO 1162作为受体菌株进行分批交配实验，以定量交配培养基中底物浓度对观察到的质粒转移速率系数的影响。交配培养基中底物浓度的影响与供体菌株的生长历史高度相关。当供体菌株在指数生长期收获时，未观察到影响;然而，当供体菌株从稳定期取出时，测定到底物浓度的强烈影响：底物浓度降低10倍，观察到的质粒转移率降低55%。
Batch mating experiments with Pseudomonas putida PAW 1 (TOL) as a donor and Pseudomonas aeruginosa PAO 1162 as a recipient strain were performed to quantify the effect of the substrate concentration in the mating medium on the observed plasmid transfer rate coefficient. The impact of the substrate concentration in the mating medium was highly correlated with the growth history of the donor strain. When the donor strain was harvested in exponential growth phase, no impact was observed; when the donor strain was taken from the stationary phase, however, a strong impact of the substrate concentration was measured: a 10‐fold reduction in the substrate concentration decreased the observed plasmid transfer rate by 55%.