Mast cell-derived mediators protect against oxygen-glucose deprivation-induced injury in PC12 cells and neurons
Mast cell-derived mediators protect against oxygen-glucose deprivation-induced injury in PC12 cells and neurons
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肥大细胞衍生的介质可保护 PC12 细胞和神经元免受缺氧葡萄糖诱导的损伤
DOI：
10.1016/j.neulet.2007.05.002
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发表时间：
2007-08-09
影响因子：
2.5
通讯作者：
Chen, Zhong
中科院分区：
文献类型：
--
作者：
Hu, Weiwei;Fan, Yanyin;Chen, Zhong
近年来的研究表明，肥大细胞在脑缺血后的病理过程中起着重要的作用。本研究通过将肥大细胞体外缺血诱导的上清加入到神经元和PC 12细胞中，在相同的条件下（氧-葡萄糖剥夺，OGD），观察肥大细胞对脑缺血后神经元损伤的影响。通过3-[4，5-二甲基噻唑-2-基]-2，5-二苯基-四唑溴化铵（MTT）测定来评估细胞损伤程度。肥大细胞衍生的上清液保护OGD诱导的PC 12细胞和神经元的损伤，并且这种保护被组胺H-2拮抗剂和抗组胺血清逆转，但不被H-2拮抗剂逆转。然而，组胺和神经生长因子（NGF）单独或。一起没有对OGD诱导的损伤的保护作用。这些结果表明，肥大细胞衍生的保护在体外缺血是组胺依赖性的，并涉及与其他介质，但不是神经生长因子的合作。(C)2007爱思唯尔爱尔兰有限公司保留所有权利。
Recent reports and our previous study suggest that mast cells play: a crucial role in the pathological processes that follow cerebral ischemia. In this study, the effect of mast cells on neuron injury after cerebral ischemia was determined by adding in vitro ischemia-induced supernatant from mast cells to neurons and PC 12 cells under the same conditions (oxygen-glucose deprivation, OGD). The degree of cell injury was evaluated by the 3-[4,5-dimethylthiazol-2-yll-2,5-dipheny-Itetrazolium bromide (MTT) assay. Mast cell-derived supernatant protected against OGD-induced injury of PC12 cells and neurons, and this protection was reversed by a histamine H, antagonist and by anti-histamine serum, but not by an H-2 antagonist. However, histamine and nerve growth factor (NGF) added separately or. together did not have protective effects against OGD-induced injury. These results indicate that mast cell-derived protection during in vitro ischemia is histamine-dependent, and involves cooperation with other mediators, but not NGF. (C) 2007 Elsevier Ireland Ltd. All rights reserved.