Chemosensory Transmembrane Protein Families in the Coffee White Stemborer, Xylotrechus quadripes (Coleoptera: Cerambycidae)
Chemosensory Transmembrane Protein Families in the Coffee White Stemborer, Xylotrechus quadripes (Coleoptera: Cerambycidae)
复制标题
咖啡白茎虫、Xylotrechus fourripes 中的化学感应跨膜蛋白家族（鞘翅目：天牛科）
DOI：
10.1093/ee/nvy076
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发表时间：
2018
影响因子：
1.7
通讯作者：
Liu Nai-Yong
中科院分区：
文献类型：
--
作者：
Pang Ji-Xin;Zeng Xin;Zhu Jia-Ying;Liu Nai-Yong
咖啡白色蛀茎虫XylotrechusquadripesChevrolat（鞘翅目：天牛科）主要取食咖啡。（龙胆目：茜草科），其卵、幼虫和蛹在树干内发育。与成虫交配、寻找宿主和识别有关的化学感觉相关线索的检测是由气味（OR）、味觉（GR）和离子型（IR）受体以及感觉神经元膜蛋白（SNMP）的三种化学感受器基因库驱动的。然而，这些基因参与化学感受的信息在X中是不可用的。天牛科昆虫中四倍体和相对贫乏。在这里，我们提出了四个化学感受跨膜蛋白的鉴定触角转录组的X。四足动物，包括33个OR，5个GR，18个IR和4个SNMP。系统发育分析将ORs分为第1、2、3、7组和嗅觉辅助受体（Orco），显示了三个潜在的候选者（OR13、OR17和OR21），用于感知雄性信息素。IR被聚集成10个直系同源群，其中还有IR 41 a、IR 64 a和IR 75进化枝的额外拷贝。四个SNMPs分布在四个独立的分支，可能代表了一个完整的集在这个物种。表达谱显示，所有的基因都在触角中高表达，这表明它们的嗅觉功能。此外，大多数基因显示在非触角组织中表达，包括胸，触角，翅膀和腿，这表明它们参与非化学感受功能。值得注意的是，一个高度保守的辅助受体IR 25 a在触角中显示出雄性偏好的表达，这是天牛中首次出现的。本研究为了解该甲虫与其寄主植物之间的相互作用机制建立了参考资源。
The coffee white stemborer,Xylotrechus quadripesChevrolat (Coleoptera: Cerambycidae), feeds primarily onCoffea arabicaL. (Gentianales: Rubiaceae) with its egg, larva, and pupa being developed within the trunk. The detection of chemosensory-related cues linked to adult mating, host seeking, and recognition is driven by three chemoreceptor gene repertoires of odorant (ORs), gustatory (GRs), and ionotropic (IRs) receptors as well as sensory neuron membrane proteins (SNMPs). Yet, information on these genes involved in chemoreception is unavailable inX. quadripesand relatively poor in the cerambycid beetles. Here, we presented the identification of four chemosensory transmembrane proteins from the antennal transcriptome ofX. quadripes, including 33ORs, fiveGRs, 18IRs, and fourSNMPs. Phylogenetic analysis classified the ORs into groups 1, 2, 3, 7, and olfactory coreceptor (Orco), showing three potential candidates (OR13, OR17, and OR21) for the sensing of male sex pheromones. The IRs were clustered into 10 orthologous groups, with additional copies for IR41a, IR64a, and IR75 clades. Four SNMPs were distributed in four independent clades, possibly representing a complete set in this species. Expression profiles revealed that all the genes were highly expressed in antennae, suggesting their olfactory roles. In addition, most of the genes showed the expression in nonantennal tissues including thoraxes, abdomens, wings, and legs, suggesting their involvement in nonchemosensory functions. Of notice, a highly conserved coreceptorIR25adisplayed male-biased expression in the antennae, as the first presence in the cerambycid beetles. This study has established reference resources for understanding the mechanisms underlying the interactions between/within this beetle and its host plants.