ANALYSIS OF WEAK REACTION SAMPLES IN REVERSED TYPING WITH COLUMN AGGLUTINATION TECHNOLOGY
ANALYSIS OF WEAK REACTION SAMPLES IN REVERSED TYPING WITH COLUMN AGGLUTINATION TECHNOLOGY
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柱凝集技术反向打字弱反应样品分析
DOI：
10.3925/jjtc1958.51.565
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
A. Ohara
中科院分区：
文献类型：
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作者：
Yoko Hidaka;N. Kawata;M. Okuda;Tadashi Kato;Tomoko Kuribayashi;Nobuya Koyama;A. Ohara
基于柱凝集技术（CAT）的自动血型检测系统没有提供反向分型的标准。如果试管法（TT）标准被引入到CAT方法的自动化中，则TT方法的确认测试频率将增加。在本研究中，我们使用自动CAT方法产生弱结果的样本（反应性<3+;AutoVue系统，Ortho）比较了CAT和TT方法的结果。采用手动CAT方法（BioVue，Ortho）孵育固定3分钟后，45%的弱样本显示凝集反应性增加。所有481份弱样本与TT方法的反应性均大于3+。80%的弱标本有IgM抗体。对于所有样品，TT法的滴定和评分结果均大于手动CAT法。对于所有样品，所有方法的弱样品滴定和评分均较低。总之，我们建议自动CAT方法中的1+反应性需要使用TT方法进行确认试验，因为CAT 2+样品可以使用TT方法显示为3+。
The automated blood group testing system based on column agglutination technology(CAT) does not provide a standard for reversed typing. If the tube test(TT)method standard is introduced into automated the CAT method, the frequency of confirmation testing with the TT method will increase. In this study, we compared the results of the CAT and TT methods using samples which produced weak results with the automated CAT method(reactivity<3+;AutoVue System, Ortho). Forty-five percent of weak samples showed an increase in agglutination reactivity after a fixed 3-minute incubation time with the manual CAT method(Bio Vue, Ortho). All 481 weak samples had greater than 3+reactivity with the TT method. Eighty percent of weak samples possessed IgM antibody. Titration and scoring results with the TT method were greater than with the manual CAT method for all samples. Titration and scoring of the weak samples were low with all methods for all samples. In conclusion, we propose that 1+reactivity in the automated CAT method requires a confirmation test with the TT method because CAT 2+samples can present as 3+with the TT method.