Fabrication and in vitro biological properties of piezoelectric bioceramics for bone regeneration.
Fabrication and in vitro biological properties of piezoelectric bioceramics for bone regeneration.
复制标题
用于骨再生的压电生物陶瓷的制备及其体外生物学性能
DOI：
10.1038/srep43360
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发表时间：
2017-02-27
影响因子：
4.6
通讯作者：
Zhao K
中科院分区：
文献类型：
--
作者：
Tang Y;Wu C;Wu Z;Hu L;Zhang W;Zhao K
生物压电材料的压电效应促进骨生长。然而，该材料应该受到应力之前，它可以产生一个电荷，促进骨修复和重建有利于骨折愈合。提出了一种新的生物压电材料生理负载体外实验方法。为了获得压电效应在骨修复中的真实表达，设计了一种模拟人体运动力的动态加载装置，并在压电材料与细胞共培养时对压电材料施加周期性载荷。采用注浆成型法制备了羟基磷灰石（HA）/钛酸钡（BaTiO 3）复合材料，通过极化测试了复合材料的压电性能。当BaTiO 3含量为80%~ 100%时，HA/BaTiO 3压电陶瓷极化后的d33为1.3 ~ 6.8 pC/N。研究了压电生物陶瓷在循环加载和非循环加载条件下的体外生物学性能。当HA/BaTiO 3压电生物陶瓷受到循环载荷的影响时，BaTiO 3的压电效应促进了成骨细胞的生长并与HA相互作用，其效果优于单独使用HA的效果。10%HA/90%BaTiO3压电陶瓷的生物相容性和骨诱导活性最好。
The piezoelectric effect of biological piezoelectric materials promotes bone growth. However, the material should be subjected to stress before it can produce an electric charge that promotes bone repair and reconstruction conducive to fracture healing. A novel method forin vitroexperimentation of biological piezoelectric materials with physiological load is presented. A dynamic loading device that can simulate the force of human motion and provide periodic load to piezoelectric materials when co-cultured with cells was designed to obtain a realistic expression of piezoelectric effect on bone repair. Hydroxyapatite (HA)/barium titanate (BaTiO3) composite materials were fabricated by slip casting, and their piezoelectric properties were obtained by polarization. Thed33of HA/BaTiO3piezoelectric ceramics after polarization was 1.3 pC/N to 6.8 pC/N with BaTiO3content ranging from 80% to 100%. Thein vitrobiological properties of piezoelectric bioceramics with and without cycle loading were investigated. When HA/BaTiO3piezoelectric bioceramics were affected by cycle loading, the piezoelectric effect of BaTiO3promoted the growth of osteoblasts and interaction with HA, which was better than the effect of HA alone. The best biocompatibility and bone-inducing activity were demonstrated by the 10%HA/90%BaTiO3piezoelectric ceramics.