Simplified Flow Cytometric Assessment in Mycosis Fungoides and Sezary Syndrome
Simplified Flow Cytometric Assessment in Mycosis Fungoides and Sezary Syndrome
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DOI：
10.1309/ajcp09otjoyavzzk
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发表时间：
2011-12-01
影响因子：
3.5
通讯作者：
Borowitz, Michael J.
中科院分区：
文献类型：
--
作者：
Hristov, Alexandra C.;Vonderheid, Eric C.;Borowitz, Michael J.
采用CD3、CD4、CD26和CD7标记的流式细胞术检测109例患者血液样本中的T细胞异常：69例蕈样真菌病(MF)/Sezary综合征（SS）患者，31例住院对照患者，9例炎症性皮肤病患者。T细胞被鉴定为定量异常（约占CD26-或CD7- T细胞的15%）或表型异常（CD26-或CD7- T细胞的CD3或CD4亮或CD7亮）。患者随访中位数为82个月，异常T细胞与诊断、临床结果和其他实验室参数相关。46%的MF/SS患者（32/69）发现t细胞群异常，并与疾病程度相关。定量异常发生率高于表型异常发生率，CD4+/CD26- T细胞发生率高于CD4+/CD7- T细胞。CD26- T细胞与疾病程度的相关性优于CD7- T细胞。异常T细胞数量的增加与疾病的恶化有关。流式细胞术为MF/SS的诊断、预后和治疗效果提供了有价值的信息。
By using flow cytometry with markers for CD3, CD4, CD26, and CD7, we examined the blood samples of 109 patients for abnormal T cells: 69 patients with mycosis fungoides (MF)/Sezary syndrome (SS), 31 hospitalized control subjects, and 9 patients with inflammatory skin disease. T cells were identified as quantitatively abnormal (> 15% CD26- or CD7- T cells) or phenotypically abnormal (CD26- or CD7- T cells with bright or dim CD3 or CD4 or bright CD 7). Patients were followed for a median of 82 months, and abnormal T cells were correlated with diagnosis, clinical outcome, and other laboratory parameters.Abnormal T-cell populations were identified in 46% of patients with MF/SS (32/69) and correlated with disease extent. Quantitative abnormalities were more frequent than phenotypic abnormalities, and CD4+/CD26- T cells were more frequent than CD4+/CD7- T cells. CD26- T cells correlated better with disease extent than did CD7-. Increasing numbers of abnormal T cells were associated with worsening disease. Flow cytometry provides valuable information for diagnosis, prognosis, and therapeutic efficacy in MF/SS.