MAST-CELL CLONES - A MODEL FOR THE ANALYSIS OF CELLULAR MATURATION
MAST-CELL CLONES - A MODEL FOR THE ANALYSIS OF CELLULAR MATURATION
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DOI：
10.1083/jcb.95.2.435
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发表时间：
1982-01-01
影响因子：
7.8
通讯作者：
DVORAK, HF
中科院分区：
文献类型：
--
作者：
GALLI, SJ;DVORAK, AM;DVORAK, HF
克隆小鼠肥大细胞的超微结构类似于体内观察到的未成熟肥大细胞。这些肥大细胞克隆可以在没有任何其他细胞的情况下生长，便于直接研究其生物化学和功能。克隆的肥大细胞表达质膜受体（FC.R），其以与正常小鼠腹膜肥大细胞相似的平衡常数（KA）结合小鼠IgF。克隆的肥大细胞不显示可检测的Ia抗原，并且当加入淋巴细胞培养物或介导自然杀伤细胞溶解时不能增强IG分泌。在1 mM丁酸钠的存在下，克隆的肥大细胞停止分裂，并获得丰富的电子致密的细胞质颗粒类似于成熟的肥大细胞。它们的组胺含量随着细胞质颗粒成熟而增加，可能超过未经处理的肥大细胞50倍。与腹膜肥大细胞不同，克隆肥大细胞将35SO4掺入硫酸软骨素而不是肝素。与完全分化的小鼠腹腔肥大细胞不同，克隆的未成熟小鼠肥大细胞不含肝素和低水平的组胺。高亲和力FC.R在肥大细胞成熟的早期表达，早在细胞质颗粒合成和介质储存完成之前。
Cloned mouse mast cells resemble, by ultrastructure, immature mast cells observed in vivo. These mast cell clones can be grown in the absence of any other cells, facilitating direct investigations of their biochemistry and function. Cloned mast cells express plasma membrane receptors (FC.epsilon.R) that bind mouse IgF with an equilibrium constant (KA) similar to that of normal mouse peritoneal mast cells. Cloned mast cells do not display detectable Ia antigens and cannot enhance Ig secretion when added to lymphocyte cultures or mediate natural killer lysis. In the presence of 1 mM sodium butyrate, cloned mast cells stop dividing and acquire abundant electron-dense cytoplasmic granules similar to those of mature mast cells. Their histamine content increases concomitant with cytoplasmic granule maturation and may exceed that of untreated mast cells by 50-fold. Unlike peritoneal mast cells, cloned mast cells incorporate 35SO4 into chondroitin sulfates rather than heparin. Unlike fully differentiated mouse peritoneal mast cells, cloned immature mouse mast cells contain no heparin and low levels of histamine. High-affinity FC.epsilon.R are expressed early in mast cell maturation, well before completion of cytoplasmic granule synthesis and mediator storage.