Genome-wide characterization of laccase gene family in Schizophyllum commune 20R-7-F01, isolated from deep sediment 2 km below the seafloor.
Genome-wide characterization of laccase gene family in Schizophyllum commune 20R-7-F01, isolated from deep sediment 2 km below the seafloor.
复制标题
从海底以下 2 公里深层沉积物中分离的裂褶菌 20R-7-F01 漆酶基因家族的全基因组特征
DOI：
10.3389/fmicb.2022.923451
复制
发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
漆酶是木质素分解酶，在丝状真菌的各种生物过程中发挥着至关重要的作用，包括子实体形成和木质素降解。木质素降解是一个复杂的过程，裂褶藻中木质素的降解很大程度上受氧气供应情况的影响。在这里，从 S. commune 20R-7-F01 基因组中总共鉴定出了六个假定的漆酶基因 (ScLAC)。这些基因包括三个典型的铜氧化酶结构域，根据系统发育分析可以分为三组。 ScLAC表现出独特的内含子-外显子结构和保守基序，表明ScLAC在基因结构上的保守性和多样性。此外，ScLAC 基因之间的顺式作用元件（例如底物利用、应激、细胞分裂和转录激活相关的顺式元件）的数量和类型有所不同，这表明 S. commune 20R-7-F01 中漆酶基因的转录可能是由不同的底物、应激或其他因素诱导的。重测序数据的SNP分析表明，栖息于海底深层沉积物的S. commune的ScLAC与栖息于陆地环境的S. commune的ScLAC存在显着差异。同样，海底菌株和陆地菌株之间漆酶的保守基序数量和排列存在巨大差异，表明 ScLAC 具有不同的结构。 ScLAC5和ScLAC6基因的表达在木质素/褐煤培养基中显着上调，表明这两个漆酶基因可能参与厌氧条件下褐煤和木质素的真菌利用和降解。这些发现可能有助于了解漆酶在白腐真菌中的功能，并为进一步探讨LAC家族与白腐真菌厌氧降解木质素之间的关系提供科学依据。
Laccases are ligninolytic enzymes that play a crucial role in various biological processes of filamentous fungi, including fruiting-body formation and lignin degradation. Lignin degradation is a complex process and its degradation in Schizophyllum commune is greatly affected by the availability of oxygen. Here, a total of six putative laccase genes (ScLAC) were identified from the S. commune 20R-7-F01 genome. These genes, which include three typical Cu-oxidase domains, can be classified into three groups based on phylogenetic analysis. ScLAC showed distinct intron-exon structures and conserved motifs, suggesting the conservation and diversity of ScLAC in gene structures. Additionally, the number and type of cis-acting elements, such as substrate utilization-, stress-, cell division- and transcription activation-related cis-elements, varied between ScLAC genes, suggesting that the transcription of laccase genes in S. commune 20R-7-F01 could be induced by different substrates, stresses, or other factors. The SNP analysis of resequencing data demonstrated that the ScLAC of S. commune inhabiting deep subseafloor sediments were significantly different from those of S. commune inhabiting terrestrial environments. Similarly, the large variation of conserved motifs number and arrangement of laccase between subseafloor and terrestrial strains indicated that ScLAC had a diverse structure. The expression of ScLAC5 and ScLAC6 genes was significantly up-regulated in lignin/lignite medium, suggesting that these two laccase genes might be involved in fungal utilization and degradation of lignite and lignin under anaerobic conditions. These findings might help in understanding the function of laccase in white-rot fungi and could provide a scientific basis for further exploring the relationship between the LAC family and anaerobic degradation of lignin by S. commune.