Forensic Toxicological Determination of Surfactant by Liquid Chromatography/Electrospray Ionization Mass Spectrometry and Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry
Forensic Toxicological Determination of Surfactant by Liquid Chromatography/Electrospray Ionization Mass Spectrometry and Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry
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液相色谱/电喷雾电离质谱和液相色谱/电喷雾串联质谱法法医毒理学测定表面活性剂
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
H. Tsuchihashi
中科院分区：
文献类型：
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作者：
M. Nishikawa;M. Katagi;A. Miki;H. Tsuchihashi
从法医毒理学的角度，通过液相色谱/电喷雾电离质谱（LC/ESI - MS）和液相色谱/电喷雾电离串联质谱（LC/ESI - MS/MS）研究了一种鉴定表面活性剂的方法。对阴离子、阳离子和非离子表面活性剂在正负两种模式下的分析表明，阴离子表面活性剂在负模式下检测为M - 离子，阳离子表面活性剂在正模式下检测为M + 离子，而非离子表面活性剂在正模式下出现[M + H]+离子或[M + NH4]+离子。阴离子表面活性剂（直链烷基苯磺酸盐和聚氧乙烯烷基醚硫酸盐）使用弱阴离子交换柱成功提取，回收率为53.8% - 76.7%。阳离子表面活性剂（苯扎氯铵）也可使用弱阳离子交换柱轻松提取，回收率为107%。当阳离子、阴离子和非离子表面活性剂的水溶液浓缩至干时，回收率在65.8%到124%之间。通过在LC/ESI - MS/MS的产物离子扫描模式下分析阴离子表面活性剂（α - 烯烃硫酸盐、聚氧乙烯烷基醚硫酸盐和直链烷基苯磺酸盐），可以确定表面活性剂亲水基团的结构，并且证实MS/MS是一种强大的分类技术。
A procedure for identifying surfactants was investigated by liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) from the forensic toxicological point of view. The analysis of anionic, cationic, and nonionic surfactants in both negative and positive mode revealed that anionic and cationic surfactants are detected as M- ions in the negative mode and M+ ions in the positive mode, respectively, while [M+H]+ ions or [M+NH4]+ ions appeared for nonionic surfactants in the positive mode. Anionic surfactants (linear alkylbenzene sulfonate and polyoxyethylene alkyl ether sulfate) were successfully extracted using a weak anion exchange cartridge, with recovery rates of 53.8-76.7%. Cationic surfactant (benzalkonium) was also easily extracted using a weak cation exchange cartridge with a recovery rate of 107%. When aqueous solutions of cationic, anionic, and nonionic surfactants were concentrated to dryness, the recovery rates ranged from 65.8% to 124%. By analyzing anionic surfactants (alpha-olefine sulfate, polyoxyethylene alkyl ether sulfate, and linear alkylbenzene sulfonate) in the product ion scan mode in LC/ESI-MS/MS, it was possible to identify the structure of the hydrophilic group of surfactants, and MS/MS was confirmed to be a powerful technique for classification.