Water-deficit inducible expression of a cytokinin biosynthetic gene IPT improves drought tolerance in cotton.
Water-deficit inducible expression of a cytokinin biosynthetic gene IPT improves drought tolerance in cotton.
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细胞分裂素生物合成基因 IPT 的缺水诱导表达可提高棉花的耐旱性
DOI：
10.1371/journal.pone.0064190
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Kuppu S;Mishra N;Hu R;Sun L;Zhu X;Shen G;Blumwald E;Payton P;Zhang H
缺水压力是限制全球农业生产力的主要环境因素。最近发生的极端干旱严重影响了美国西南部的棉花产量。迫切需要开发耐缺水胁迫能力更强的棉花品种，以便在缺水地区实现可持续生产。一种设计抗旱性的方法是通过上调细胞分裂素的生物合成来延缓干旱诱导的衰老。将编码细胞分裂素生物合成限速酶的异戊烯基转移酶基因(IPT)导入棉花，并在温室和温室条件下分析了转PSARK：：IPT基因棉花植株的表现。这些数据表明，转PSARK：：IPT基因棉花植株在温室缺水条件下表现出延缓衰老的现象。与野生型和分离的非转基因品系相比，这些植株在减少灌溉条件下产生了更多的根和地上部分生物量，减少了落花，保持了更高的叶绿素含量和更高的光合作用速率。此外，在生长室条件下生长的转PSARK：：IPT基因棉花植株也表现出更强的抗旱性。这些结果表明，水分亏缺诱导棉花异戊烯基转移酶基因的表达可以显著提高棉花的抗旱性。
Water-deficit stress is a major environmental factor that limits agricultural productivity worldwide. Recent episodes of extreme drought have severely affected cotton production in the Southwestern USA. There is a pressing need to develop cotton varieties with improved tolerance to water-deficit stress for sustainable production in water-limited regions. One approach to engineer drought tolerance is by delaying drought-induced senescence via up-regulation of cytokinin biosynthesis. The isopentenyltransferase gene (IPT) that encodes a rate limiting enzyme in cytokinin biosynthesis, under the control of a water-deficit responsive and maturation specific promoter PSARK was introduced into cotton and the performance of the PSARK::IPT transgenic cotton plants was analyzed in the greenhouse and growth chamber conditions. The data indicate that PSARK::IPT-transgenic cotton plants displayed delayed senescence under water deficit conditions in the greenhouse. These plants produced more root and shoot biomass, dropped fewer flowers, maintained higher chlorophyll content, and higher photosynthetic rates under reduced irrigation conditions in comparison to wild-type and segregated non-transgenic lines. Furthermore, PSARK::IPT-transgenic cotton plants grown in growth chamber condition also displayed greater drought tolerance. These results indicate that water-deficit induced expression of an isopentenyltransferase gene in cotton could significantly improve drought tolerance.
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影响因子：
56.9
作者：
BOYER, JS
通讯作者：
BOYER, JS
影响因子：
9.4
作者：
Galmes, Jeroni;Medrano, Hipolito;Flexas, Jaume
通讯作者：
Flexas, Jaume
DOI：
10.1051/agro:2008021
发表时间：
2009-01-01
期刊：
SUSTAINABLE AGRICULTURE
影响因子：
--
作者：
Farooq, M.;Wahid, A.;Basra, S. M. A.
通讯作者：
Basra, S. M. A.
影响因子：
6.4
作者：
HE, JX;WANG, J;LIANG, HG
通讯作者：
LIANG, HG
影响因子：
56.9
作者：
GAN, SS;AMASINO, RM
通讯作者：
AMASINO, RM