An experimental study of ovarian cancer imaging and therapy by paclitaxel-loaded phase-transformation lipid nanoparticles combined with low-intensity focused ultrasound
An experimental study of ovarian cancer imaging and therapy by paclitaxel-loaded phase-transformation lipid nanoparticles combined with low-intensity focused ultrasound
复制标题
紫杉醇相变脂质纳米粒联合低强度聚焦超声显像及治疗卵巢癌的实验研究
DOI：
10.1016/j.bbrc.2018.08.156
复制
发表时间：
2018-09-26
影响因子：
3.1
通讯作者：
Zhu, Yuanfang
中科院分区：
文献类型：
--
作者：
Gao, Yanling;Chen, Shaoyun;Zhu, Yuanfang
载药相变脂质纳米粒（NPs）与低强度聚焦超声（LIFU）结合用于超声分子成像和治疗，是一种很有前途的药物载体，可同时提供物理和化学治疗。我们成功地制备了紫杉醇（PTX）靶向脂质纳米粒在本研究中，PTX-抗-LHRHR-PTNP联合LIFU治疗卵巢癌具有以下特点：一方面，它表现出比血细胞更小的体积和更大的稳定性，这显著延长了它在体内的半衰期，并且可以主动靶向卵巢癌OVCAR-3细胞，并顺利穿透内皮间隙进入肿瘤部位，特异性杀伤卵巢癌细胞。从而提高了治疗效果，降低了毒副作用，最大限度地保护了正常组织，减少了不良反应。另一方面，PTX-抗-LHRHR-PTNP在静脉内注射后可靶向至病灶，并在肿瘤靶组织中长时间保留。同时，在LIFU触发下可发生液-气相变，从而产生更理想和持续的超声成像效果。然后利用声学造影剂对超声散射进行分子水平的显影，从而从分子水平对病变组织进行评价。(C)2018爱思唯尔公司All rights reserved.
Drug-loaded phase-transformation lipid nanoparticles (NPs) combined with low-intensity focused ultrasound (LIFU) for ultrasound molecular imaging and therapy, which is a very promising drug carrier and can provide both physical and chemical therapeutics, simultaneously. We successfully prepared the paclitaxel (PTX) loaded anti-LHRHR targeted phase-transformation lipid nanoparticles (PTX-anti-LHRHR-PTNPs) for ovarian cancer in this study combined with LIFU has the following characteristics: On the one hand, it showed smaller size and greater stability than blood cells, which significantly prolonged its half-life in the body, and can actively target ovarian cancer OVCAR-3 cells, and smoothly penetrate the endothelial gap into the tumor site for specifically killing the ovarian cancer cells. Thereby, the special drug carrier improved the therapeutic effect and reduced toxic and side effects, maximized the protection of normal tissues and minimized adverse reactions. On the other hand, PTX-anti-LHRHR-PTNPs can be targeted to focus after being injected intravenously and remain in the tumor target tissue for a long time. At the same time, liquid-gas phase-transformation can occur under LIFU triggering, resulting in more ideal and sustained ultrasound imaging effects. Then acoustic contrast agent is used to develop the molecular level of ultrasound scattering, so as to evaluate the diseased tissue from the molecular level. (C) 2018 Elsevier Inc. All rights reserved.