Development of SCAR markers linked to powdery mildew (Uncinula necator) resistance in grapevine (Vitis vinifera L. and Vitis sp.)
Development of SCAR markers linked to powdery mildew (Uncinula necator) resistance in grapevine (Vitis vinifera L. and Vitis sp.)
复制标题
DOI：
10.1007/s11032-006-9047-9
复制
发表时间：
2007-02-01
影响因子：
3.1
通讯作者：
Zyprian, Eva
中科院分区：
文献类型：
--
作者：
Akkurt, Murat;Welter, Leocir;Zyprian, Eva
利用6个随机扩增多态性DNA（RAPD）标记，在葡萄藤品种“Regent”（抗病）דLemberger”（感病）杂交的试验群体中构建了与白粉病抗性主效QTL连锁的序列特征扩增区域标记（SCARs）。对RAPD扩增产物进行克隆和测序。设计引物长度至少为21个核苷酸的引物对。在“Regent”X“Lemberger”的F1后代中测试所有对。SCAR引物扩增出预期大小的特异性条带，并在抗性和感病种质的其他遗传资源中进行了测试。所有的SCAR引物对均扩增出特异性片段。其中两个SCAR标记ScORA 7 -760和ScORN 3-R主要在抗性个体中产生扩增产物，并被发现与抗病性相关。ScORA 7 -760特别适用于白粉病抗性的标记辅助选择，并有助于从各种来源中筛选白粉病抗性基因。
Sequence-characterized amplified regions markers (SCARs) were developed from six randomly amplified polymorphic DNA (RAPD) markers linked to the major QTL region for powdery mildew (Uncinula necator) resistance in a test population derived from the cross of grapevine cultivars "Regent" (resistant) x "Lemberger"(susceptible). RAPD products were cloned and sequenced. Primer pairs with at least 21 nucleotides primer length were designed. All pairs were tested in the F1 progeny of "Regent" x "Lemberger". The SCAR primers resulted in the amplification of specific bands of expected sizes and were tested in additional genetic resources of resistant and susceptible germplasm. All SCAR primer pairs resulted in the amplification of specific fragments. Two of the SCAR markers named ScORA7-760 and ScORN3-R produced amplification products predominantly in resistant individuals and were found to correlate to disease resistance. ScORA7-760, in particular, is suitable for marker-assisted selection for powdery mildew resistance and to facilitate pyramiding powdery mildew resistance genes from various sources.