MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis.
MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis.
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DOI：
10.3389/fmicb.2015.00791
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发表时间：
2015
影响因子：
5.2
通讯作者：
Virdi JS
中科院分区：
文献类型：
--
作者：
Singhal N;Kumar M;Kanaujia PK;Virdi JS
目前，微生物最好使用16 S rRNA和18 S rRNA基因测序来鉴定。然而，近年来，基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）已成为微生物鉴定和诊断的潜在工具。在MALDI-TOF MS过程中，使用完整细胞或细胞提取物鉴定微生物。该方法快速、灵敏，并且在所涉及的劳动力和成本方面经济。该技术已经很容易被微生物学家吸收，他们已经报告了MALDI-TOF MS用于多种目的，如微生物鉴定和菌株分型、流行病学研究、生物战剂检测、水和食源性病原体检测，抗生素耐药性检测和血液和泌尿道病原体检测等。该技术的局限性在于可能鉴定新的分离株仅当光谱数据库包含特定属/种/亚种/菌株的模式菌株的肽质量指纹时。本文综述了质谱技术在微生物鉴定中的应用现状。它还探讨了这种令人兴奋的新技术在诊断由细菌，病毒和真菌引起的疾病方面的实用性。
Currently microorganisms are best identified using 16S rRNA and 18S rRNA gene sequencing. However, in recent years matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a potential tool for microbial identification and diagnosis. During the MALDI-TOF MS process, microbes are identified using either intact cells or cell extracts. The process is rapid, sensitive, and economical in terms of both labor and costs involved. The technology has been readily imbibed by microbiologists who have reported usage of MALDI-TOF MS for a number of purposes like, microbial identification and strain typing, epidemiological studies, detection of biological warfare agents, detection of water- and food-borne pathogens, detection of antibiotic resistance and detection of blood and urinary tract pathogens etc. The limitation of the technology is that identification of new isolates is possible only if the spectral database contains peptide mass fingerprints of the type strains of specific genera/species/subspecies/strains. This review provides an overview of the status and recent applications of mass spectrometry for microbial identification. It also explores the usefulness of this exciting new technology for diagnosis of diseases caused by bacteria, viruses, and fungi.