Tuberculosis caused by Mycobacterium orygis in wild ungulates in Chennai, South India
Tuberculosis caused by Mycobacterium orygis in wild ungulates in Chennai, South India
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DOI：
10.1111/tbed.14613
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发表时间：
2022-06-25
影响因子：
4.3
通讯作者：
Palaniyandi, Kannan
中科院分区：
文献类型：
--
作者：
Refaya, Ahmed Kabir;Ramanujam, Harini;Palaniyandi, Kannan
我们报告了从印度钦奈的Guindy国家公园森林范围内的两只黑巴克斯（羚羊cervicapra）和一只梅花鹿（Axis axis）中分离出结核分枝杆菌复合体（MTBC）的成员--稻分枝杆菌。采用NaOH法处理尸检期间采集的肺组织和淋巴结样本，并在固体和液体培养基中培养。从培养的分离物中提取的DNA用于通过特异性引物扩增mpt 64基因，并且在240 bp处可见的条带证实分离物是MTBC的成员。通过spoligotyping和全基因组测序进一步检查这些分离株，证实分离株为M。orygis的系统发育树显示它们与其它M.在地球仪上分离出的。RD 7-RD 10、RDOryx_1、RDOryx_4、RD 12 Oryx、RD 301和RD 315的缺失进一步证实了这些分离物为M.奥瑞吉斯动物感染的确切来源无法追踪，基于单核苷酸多态性差异的基因组成对比较未检测到受影响动物内的任何传播事件。然而，考虑到由于人与动物之间的互动增加而存在高传播机会的环境是明智的。由于众所周知，病原体能够在人类和动物宿主中引起感染，因此对梅花鹿、黑雄鹿以及附近的人类进行系统的监测和筛查对于成功实施“一个健康”方法至关重要。
We report the isolation of Mycobacterium orygis, a member of Mycobacterium tuberculosis complex (MTBC), from two black bucks (Antelope cervicapra) and one spotted deer (Axis axis) from the Guindy National Park forest range in Chennai, India. Lung tissue and lymph node samples collected during post-mortem examination were processed using NaOH method and cultured in solid and liquid media. DNA extracted from the cultured isolates was used to amplify the mpt64 gene by specific primers and the band visualized at 240 bps confirmed the isolates as a member of MTBC. Further examination of these isolates by spoligotyping and whole-genome sequencing confirmed the isolates as M. orygis and the phylogenetic tree revealed their well-clustered position with other M. orygis isolates around the globe. The deletion of RD7-RD10, RDOryx_1, RDOryx_4, RD12Oryx, RD301 and RD315 further substantiated these isolates as M. orygis. The exact source of infection in animals was untraceable and the pairwise comparison of the genomes based on single-nucleotide polymorphisms difference did not detect any events of transmission within the affected animals. Nevertheless, it would be wise to take into account the environment where there exists a high chance of transmission due to the increased human-animal interaction. Since it is well known that the pathogen is capable of causing infection in both human and animal hosts, systematic surveillance and screening of spotted deer, black buck as well as humans in the vicinity is essential for successful implementation of the One Health approach.