Characterisation of oligosaccharides from Drosophila melanogaster glycoproteins.
Characterisation of oligosaccharides from Drosophila melanogaster glycoproteins.
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果蝇糖蛋白寡糖的表征。
DOI：
10.1016/0304-4165(91)90245-c
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
David R. Roberts
中科院分区：
文献类型：
--
作者：
P. Williams;M. Wormald;R. Dwek;T. Rademacher;G. Parker;David R. Roberts
对果蝇三龄幼虫膜糖蛋白制剂(3rdIL)和提纯的果蝇血清蛋白2(LSP2)释放的寡糖进行了分析。序列外糖苷酶消化结合高分辨凝胶渗透层析和部分乙酰解表明存在两个系列的寡甘露糖苷，其中一个系列是罕见的，其特征是核心α1-6连接岩藻糖的存在，另一个系列是典型的哺乳动物低聚糖苷系列，包含以下异构体−D1、−D2、−D12、−D123和−CD123以及未加工的Man9GlcNAc2结构。常规寡核苷酸只能在LSP2样品中检测到。这项研究为利用强大的分子和经典遗传技术来分析高等生物中糖基化的控制和功能意义开辟了道路。
An analysis of the released oligosaccharides from a membrane glycoprotein preparation of third instar larvae (3rdIL), and purified larval serum protein 2 (LSP2) fromDrosophila melanogasterwas performed. Sequential exoglycosidase digestion in combination with high-resolution gel permeation chromatography and partial acetolysis indicated the presence of two series of oligomannosides; one of these series was unusual and characterized by the presence of a core α1–6 linked fucose, the other was a typical mammalian oligomannose series containing the following isomers −D1, −D2, −D12, −D123and −CD123as well as the unprocessed Man9GlcNAc2structure. Conventional oligomannose could only be detected in the LSP2 sample. This study opens the way to use powerful molecular and classical genetic techniques to analyse the control and functional significance of glycosylation in higher organisms.
天冬酰胺连接的寡糖加工的调节。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hsieh,P;Robbins,PW
通讯作者：
Robbins,PW
鳞翅目昆虫（草地贪夜蛾）细胞表达人纤溶酶原 cDNA 时的低聚糖加工。
影响因子：
2.9
作者：
Davidson,DJ;Fraser,MJ;Castellino,FJ
通讯作者：
Castellino,FJ