Specific binding of IL-8 to rabbit alpha-macroglobulin modulates IL-8 function in the lung.
Specific binding of IL-8 to rabbit alpha-macroglobulin modulates IL-8 function in the lung.
复制标题
IL-8 与兔 α-巨球蛋白的特异性结合可调节肺中 IL-8 的功能。
DOI：
10.1007/s000110050636
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Teodorescu,M
中科院分区：
文献类型：
--
作者：
Kurdowska,A;Fujisawa,N;Peterson,B;Carr,FK;Noble,JM;Alden,SM;Miller,EJ;Teodorescu,M
Objective and Design:The purpose of this study was to compare chemotactic activity of IL-8 alone with that of IL-8 reacted with rabbitα-macroglobulins (αM) in vivo.¶Methods:Initially the binding of recombinant human IL-8 (rhIL-8) to rabbitαM was studied.125I-labeled rhIL-8 was incubated withαM, and electrophoresed on native 5% gels or SDS-polyacrylamide 4-20% gradient gels. Next, rhIL-8 or rhIL-8 bound toαM was administered via an endotracheal tube to rabbit's lungs.¶Treatment:An endotracheal tube was wedged into a segment of the lobe of each lung, and a sample instilled through the tube into this segment. After 4 h the lungs were lavaged.¶Results:rhIL-8 bound toαM retained its full chemotactic activity in vitro but exhibited a diminished ability to induce the influx of neutrophils into the rabbit lung.¶Conclusions:The data suggest thatαM may facilitate IL-8 clearance from the lung.
