Sutureless repair of corneal injuries using naturally derived bioadhesive hydrogels
Sutureless repair of corneal injuries using naturally derived bioadhesive hydrogels
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DOI：
10.1126/sciadv.aav1281
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发表时间：
2019-03-01
期刊：
影响因子：
13.6
通讯作者：
Annabi, Nasim
中科院分区：
文献类型：
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作者：
Sani, Ehsan Shirzaei;Kheirkhah, Ahmad;Annabi, Nasim
角膜损伤是世界范围内视力损害的常见原因。因此，对于具有高粘附性、内聚性和再生性质的透明生物材料存在未满足的需求。在此，我们设计了一种高度生物相容性和透明的生物粘合剂，用于使用可见光交叉照射的天然衍生聚合物GelCORE（角膜再生凝胶）进行角膜重建。通过改变预聚物浓度和光交联时间，可对凝胶芯的物理性能进行微调。与市售粘合剂相比，GelCORE显示出更高的组织粘附性。此外，GelCORE的原位光聚合促进了向角膜的容易递送，允许根据缺损的所需几何形状精确地进行生物粘附固化。使用兔基质缺损模型的体内实验表明，生物粘附剂可以有效地密封角膜缺损并诱导基质再生和再上皮化。总的来说，GelCORE具有许多优点，包括成本低，易于生产和使用。这使得GelCORE成为一种有前途的角膜修复生物粘合剂。
Corneal injuries are common causes of visual impairment worldwide. Accordingly, there is an unmet need for transparent biomaterials that have high adhesion, cohesion, and regenerative properties. Herein, we engineer a highly biocompatible and transparent bioadhesive for corneal reconstruction using a visible light cross-linkable, naturally derived polymer, GelCORE (gel for corneal regeneration). The physical properties of GelCORE could be finely tuned by changing prepolymer concentration and photocrosslinking time. GelCORE revealed higher tissue adhesion compared to commercial adhesives. Furthermore, in situ photopolymerization of GelCORE facilitated easy delivery to the cornea, allowing for bioadhesive curing precisely according to the required geometry of the defect. In vivo experiments, using a rabbit stromal defect model, showed that bioadhesive could effectively seal corneal defects and induce stromal regeneration and re-epithelialization. Overall, GelCORE has many advantages including low cost and ease of production and use. This makes GelCORE a promising bioadhesive for corneal repair.